| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 34289894
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Nakayama K, Ikadai H |
| Name Group/Department | Laboratory of Veterinary Parasitology, School of Veterinary Medicine |
| Name Institute | Kitasato University |
| City | Towada, Aomori |
| Country | Japan |
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| Name of the mutant parasite |
| RMgm number | RMgm-5045 |
| Principal name | OSCP-KO |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Normal numbers of ookinetes are formed with wild-type morphology. Evidence is provided for reduced gliding motility of ookinetes. |
| Oocyst | Evidence is provided for reduced gliding motility of ookinetes. Reduced oocyst production. On days 9 and 14, oocyst diameter was significantly smaller than of wild-type oocysts. Transmission electron microscopy provided evidence that on day 17 oocysts exhibited wall thinning and indistinguishable internal structures (however, sporozoites are formed; see below). |
| Sporozoite | When mosquitoes were allowed to feed on naive mice 25 days after an infectious blood meal, they transmitted both WT and KO parasites, suggesting that OSCP is not essential for sporozoite function. |
| Liver stage | When mosquitoes were allowed to feed on naive mice 25 days after an infectious blood meal, they transmitted both WT and KO parasites, suggesting that OSCP is not essential for sporozoite function. |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of OSCP
Protein (function)
OSCP (oocyst capsule protein) is predicted to have 4236 amino acids and has a molecular weight of 494 kDa. It has a predicted transmembrane domain in amino acids 97–119.
Phenotype
Normal numbers of ookinetes are formed with wild-type morphology. Evidence is provided for reduced gliding motility of ookinetes. Reduced oocyst production. On days 9 and 14, oocyst diameter was significantly smaller than of wild-type oocysts. Transmission electron microscopy provided evidence that on day 17 oocysts exhibited wall thinning and indistinguishable internal structures (however, sporozoites are formed; see below).
When mosquitoes were allowed to feed on naïve mice 25 days after an infectious blood meal, they transmitted both WT and KO parasites, suggesting that OSCP is not essential for sporozoite function.
Additional information
The IFA images comparing ookinetes fixed with PFA and TritonX-100 treatment, fixed with PFA and no TritonX-100 treatment, and unfixed (live) parasites provided evidence that OSCP was expressed on the ookinete surface. PbCap380 is a surface protein of the oocyst capsule, and the IFAs of day 15 oocysts using anti-OSCP and anti-PbCap380 antibodies indicated that OSCP colocalizes with PbCap380.
Other mutants |
*RMgm-5045
