RMgmDB - Rodent Malaria genetically modified Parasites


Malaria parasiteP. berghei
DisruptedGene model (rodent): PBANKA_1427700; Gene model (P.falciparum): PF3D7_0809700; Gene product: RuvB-like helicase 1 (RUVB1, RUVBL1, RvBL1)
Phenotype Gametocyte/Gamete;
Last modified: 7 July 2021, 12:20
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 34119684
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherYuda M, Nishi T
Name Group/DepartmentDepartment of Medical Zoology
Name InstituteMie University School of Medicine
CityMie, Tsu
Name of the mutant parasite
RMgm numberRMgm-5031
Principal nameRvBL1(-)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Asexual blood stageNot different from wild type
Gametocyte/GameteTwo independent parasite clones, in which the RvBL1 gene was disrupted produced mature-appearing females and males.
However, both clones lacked exflagellation.
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

The mutant lacks expression of RvBL1 (RUVB1)

Protein (function)
In P. yoelii the CCR4-NOT complex is localized in cytoplasmic granules and is involved in translational regulation, playing important roles in development of male and female gametocytes. TRiC and the R2TP (Rvb1–Rvb2–Tah1–Pih1) complex play roles in protein synthesis as chaperones. TRiC is a molecular chaperone that is required for folding of proteins such as actin and tubulin. The R2TP complex is a co-chaperone of HSP90 that assists client proteins to form a large protein complex. Studies have demonstrated that the R2TP complex is essential for the preassembly of dynein arms, an essential step for cilium/flagellum formation.


Two independent parasite clones, in which the RvBL1 gene was disrupted produced mature-appearing females and males. However, both clones lacked exflagellation.

Additional information

From the Abstract:
'Fluorescence microscopy showed that AP2-G expression was first observed in the parasite 12 h after erythrocyte invasion and peaked at 18 h when sexual features were first manifested in  early gametocytes. Expression of  AP2-G decreased with manifestation of  sex-specific features. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed at peak AP2-G expression and identified over 1000 binding sites in the genome. The main binding motif of the TF predicted from the binding sites was GTACNY. Predicted targets contained a number of genes related to protein biogenesis, suggesting that AP2-G plays a role in establishing a cellular basis required for sexual differentiation. AP2-G binding sites also existed upstream of gametocyte-specific TFs, namely AP2-G2, AP2-FG, and AP2-G itself. Furthermore, the target contained two AP2 TF-related genes. Disruption of  these genes resulted in  the arrest of  ookinete development. These results suggest another role of  AP2-G: activating a  transcriptional cascade to  promote conversion into early gametocytes.'

Other mutants

  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1427700
Gene Model P. falciparum ortholog PF3D7_0809700
Gene productRuvB-like helicase 1
Gene product: Alternative nameRUVB1, RUVBL1, RvBL1
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markerunknown
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6