Additional remarks phenotype | Mutant/mutation
The mutant expresses of C-terminal, 6x-HA tagged version of AP2-O3.
The gene has been tagged using CRISPR/cas9 genome editing (see mutant RMgm-1096 for details).
Protein (function)
Most sequence-specific transcription factor families found in other eukaryotes seem to be absent from Plasmodium. Instead, an expansion of a protein family containing one or more apetala2 (AP2) DNA-binding domains was observed across the phylum apicomplexa. In total, 27 members of this family have been found in the human malaria parasite Plasmodium falciparum (although a possible 28th member of the family may be present. In total, 26 of these have syntenic orthologs in rodent malaria species, each with its unique stage-specific expression profile.
From the paper: ' Recently, Modrzynska et al and our group systematically investigated the functions of ApiAP2 TFs in the rodent malaria parasite P. berghei and P. yoelii, respectively (Modrzynska et al, 2017; Zhang et al, 2017b). Both studies revealed that AP2-O3, an ApiAP2 member, is expressed in the gametocytes and essential for the ookinete formation and mosquito transmission (Modrzynska et al, 2017; Zhang et al, 2017b). However, the underlying mechanism is unknown. In this study, we established AP2-O3 as a transcription repressor of the male-associated genes in the female gametocytes, which is essential for maintaining the femalespecific transcriptome.
Phenotype
Tagged AP2-O3 expressed in the nucleus of female gametocytes (not in males) and in mature oocysts (not in ookinetes, early stage oocysts and sporozoites).
Additional information
The gene has been disrupted using CRISPR/cas9 genome editing (using constructs as described for mutant RMgm-1095).
In the paper mutants are described that express mScarlet-tagged AP2-O3, 4xcmyc tagged AP2-O3 and N-terminal, 6x-HA-tagged AP2-O3
Other mutants
see ApiAP2
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