SummaryRMgm-4923
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene disruption |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 33222390 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone | P. berghei ANKA 2.34 |
Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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The mutant parasite was generated by | |
Name PI/Researcher | Liu F, Cao Y |
Name Group/Department | Department of Immunology, College of Basic Medical Sciences |
Name Institute | China Medical University |
City | Shenyang, Liaoning |
Country | China |
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Name of the mutant parasite | |
RMgm number | RMgm-4923 |
Principal name | Pb22-KO |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Normal numbers of mature male and female gametocytes are produced. Pb22-KO male gametocytes showed a ~89% reduction in the exflagellation centers formed. Normal formation (and egress) of female gametes. |
Fertilization and ookinete | Strongly reduced ookinete formation |
Oocyst | No oocyst formation |
Sporozoite | Not tested |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation Phenotype Additional information
Immunofluorescence analyses showed the following: In the erythrocytic stages, Pb22 expression was restricted to the parasites in schizonts and gametocytes. Abundant expression of the Pb22 protein in both male and female gametocytes. In the exflagellating male gametes, the protein was observed on the residual body as well as the flagella. Evidence presented for surface location in gametes and ookinetes. Other mutants |
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Details of the target gene | |||||||||||||||||||||||||
Gene Model of Rodent Parasite | PBANKA_0305900 | ||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_0208800 | ||||||||||||||||||||||||
Gene product | conserved Plasmodium protein, unknown function | ||||||||||||||||||||||||
Gene product: Alternative name | pb22 | ||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||
Type of plasmid/construct used | (Linear) plasmid double cross-over | ||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||
Partial or complete disruption of the gene | Complete | ||||||||||||||||||||||||
Additional remarks partial/complete disruption | |||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | hdhfr/yfcu | ||||||||||||||||||||||||
Promoter of the selectable marker | eef1a | ||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||
Additional remarks genetic modification | Purified and digested PCR fragments (the 5’ and 3’ recombination fragments) were cloned into the PL0034 vector | ||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
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