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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1037800
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Gene Model P. falciparum ortholog |
PF3D7_1404300
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Gene product | secreted ookinete adhesive protein, putative |
Gene product: Alternative name | SOAP |
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Details of the genetic modification |
Name of the tag | APEX2 + cmyc |
Details of tagging | C-terminal |
Additional remarks: tagging | |
Commercial source of tag-antibodies | |
Type of plasmid/construct | (Linear) plasmid single cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | soap::APEX2: The APEX2::myc sequence flanked with the restriction sites BamHI and XbaI was ordered from geneart (Regensburg, Germany). It was ligated into the previously described BioID plasmid (Kehrer, Frischknecht, et al., 2016) replacing the bira*-sequence. The resulting plasmid pL8 was linearized with HpaI prior transfection for single crossover integration |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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