Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene tagging
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Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 32736136 |
MR4 number |
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Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
P. berghei ANKA 2.34
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Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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The mutant parasite was generated by |
Name PI/Researcher | Tremp AZ, Dessens JT |
Name Group/Department | Department of Infection Biology, Faculty of Infectious and Tropical Diseases |
Name Institute | London School of Hygiene & Tropical Medicine |
City | London |
Country | UK |
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Name of the mutant parasite |
RMgm number | RMgm-4857 |
Principal name | CPW1-GFP |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype |
Asexual blood stage | Not tested |
Gametocyte/Gamete | Not tested |
Fertilization and ookinete | PBANKA_0943400 (CPW1) displayed weak GFP signal with a crystalloid-like distribution in ookinetes |
Oocyst | Not tested |
Sporozoite | Not tested |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of CPW1
Protein (function)
The protein CPW1 was identified in a GFP-affinity pulldown with high accuracy mass spectrometry using the GFP-tagged ookinete crystalloid protein LAP3 (PBANKA_0204500).
This protein is part of the nine-member ‘CPW-WPC’ domain-containing protein family
Phenotype
PBANKA_0943400 (CPW1) displayed weak GFP signal with a crystalloid-like distribution in ookinetes
Additional information
From the paper:
Our analysis identified a further two proteins, namely PBANKA_1352500 and 1346300, which were previously reported to be crystalloid-resident by GFP tagging. These two proteins are part of the nine-member ‘CPW-WPC’ domain-containing protein family. Interestingly, an additional four members of this family were identified by our analysis of the LAP3 interactome, namely PBANKA_0943400, 1015400, 1449300 and 1218300. We generated GFP-tagged parasite lines for the latter four CPW-WPC proteins, which revealed that PBANKA_0943400 and 1015400, too, displayed weak GFP signal with a crystalloid-like distribution in ookinetes. Although we could not detect discernible GFP signal in ookinetes of the other two parasite lines, possibly due to low expression levels, our findings indicate that at least several CPW-WPC family members are involved with the crystalloid.
Other mutants |