Mutant/mutation
The mutant expresses a mutated PBANKA_0112100, in which T-to-C transition at position 5468 is introduced. This mutation results resulted in a change of a phenylalanine (F) into a serine (S) (5,467 TTT to TCT). 

Protein (function)
See RMgm-4823 for details
PBANKA_0112100 is an essential transcription factor and therefore no viable knock out could be generated.

Phenotype
We compared infections by the mutant parasites PbNK65(F) (RMgm-4823) and PbANKA(S) in C57BL/6 mice to infections with their WT counterparts PbNK65(S) and PbANKA(F)

Evidence is presented that:
- PbNK65(F) does not induce ECM
- PbANKA(S) induces ECM (like wild type PbANKA(F) parasites

Additional information
PBANKA_0112100 is an essential transcription factor and therefore no viable knock out could be generated. See also RMgm-4823 for more details for these studies

From the Abstract: 
The single nucleotide polymorphysim (SNP) in the ApiAP2 gene PBANKA_0112100 is neither necessary nor sufficient to induce ECM and thus cannot account for parasite strain-specific (ANKA versus NK65) differences in ECM phenotypes.

From the paper: 
In contrast, a highly related strain to PbANKA, PbNK65, causes severe anemia in the absence of any detectable brain pathology (ECM). A comparison of high coverage genomic sequences of PbANKA and PbNK65 revealed that these two strains differ by only 21 single nucleotide polymorphisms (SNPs) in their coding regions. Thus, remarkably, a small number of SNPs may account for the dramatically different disease outcomes of infection with PbANKA versus PbNK65. The majority of the genes containing SNPs are of unknown function, however, two SNPs were identified in genes encoding proteins belonging to ApiAP2 transcriptional factor (TF) family namely PBANKA_0112100 and PBANKA_1415700. The SNP in PBANKA_1415700 was located immediately before the stop codon and hence unlikely to induce structural alterations that would lead to functional differences in the expressed ApiAP2. On the other hand, the SNP in PBANKA_0112100 was located in the predicted DNA-binding domain of ApiAP2 resulting in a substitution of a Serine (S) to Phenylalanine (F) at amino acid 1823 in the expressed protein, two biochemically distinct amino acids. Therefore, this SNP had the potential to influence the function of ApiAP2.
Our previous genetic and functional analysis using this SNP (RMgm-4823) in order to dissect out the function of this transcription factor revealed that the SNP in the DNA binding region of PBANKA_0112100 alters the expression of 46 Plasmodium genes. Among these 46 genes 39 belong to either BIR(22/46), fam-a (7/46), fam-b ( 8/46) or fam-c (2/46) gene families all of which are known to be involved in virulence and evasion related functions