Mutant/mutation
The mutant expresses a C-terminal GFP-tagged T PYYM_0017400 (in the P. yoelii 17X annotation this gene is PY17X_1100077).

Protein (function)
The ID of PIR gene PYYM_0017400 in the P. yoelii 17X annotation is PY17X_1100077.
The protein is a member of a variant antigen family (Plasmodium interspersed repeat proteins, PIR). These proteins are potentially implicated in host immune system interactions and targeted to or on the surface of the host erythrocyte. In P. vivax there are approximately 350 pir (vir) in the genome, and homologues have been identified in Plasmodium infecting rodents and monkeys; P. berghei (bir), P. chabaudi (cir), P. yoelii (yir), P. knowlesi (kir). PIR proteins show homology to proteins which are members of the RIF/STEVOR family of P. falciparum proteins. Consistent with a role in antigenic variation and immune evasion, PIR proteins have been demonstrated on, or close to the surface membrane of erythrocytes infected with P. vivax, P. chabaudi, P. yoelii and P.berghei  and switching of yir transcription has been observed in response to a host immune response.

Phenotype
From the paper: ' We tagged the PLASMED export signal motif containing YIR (PYYM_0017400) with GFP. 
The correct expression and complete ribosomal cleavage of the PYYM_0017400.GFP fusion protein was established using Western blot analysis of saponin lysed trophozoites. A 63 kDa band corresponding to the full-length PYYM_0017400.GFP fusion protein was detected in both the supernatant and the pellet, indicating that the protein was exported into the host cell cytoplasm (HCC). Live fluorescent microscopy indicated that in trophozoite-stage parasites PYYM_0017400.GFP is located in the PVM as well as in punctate dots in the HCC while in late schizonts the protein is surrounding individual merozoites. All the transfected parasites express the GFP tagged YIR.'

Additional information

Other mutants