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Details of the target gene |
Gene Model of Rodent Parasite |
py17X_1354300
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Gene Model P. falciparum ortholog |
Not available
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Gene product | conserved Plasmodium protein, unknown function |
Gene product: Alternative name | |
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Details of the genetic modification |
Name of the tag | GFP |
Details of tagging | C-terminal |
Additional remarks: tagging | |
Commercial source of tag-antibodies | |
Type of plasmid/construct | (Linear) plasmid single cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | hdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | Reverse genetic modification of P. yoelii parasites. Plasmodium yoelii (17XNL strain) was genetically modified using conventional, double homologous recombination approaches with the pDEF plasmid vector. The 3′ end of py17X_1354300 or pyuis12 (PY17X_0507300) was modified by the addition of the GFPmut2 coding sequence prior to the stop codon. Transgenic parasites were identified by genotyping PCR, with independent transgenic clones being isolated by limiting dilution cloning. Clonal parasites were transmitted to A. stephensi mosquitoes to produce salivary gland sporozoites. |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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