SummaryRMgm-4682
|
Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene disruption |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 31634979 |
MR4 number | |
top of page | |
Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei NK65 |
Name parent line/clone | Not applicable |
Other information parent line | |
top of page | |
The mutant parasite was generated by | |
Name PI/Researcher | Depoix D, Kohl L |
Name Group/Department | CNRS Molécules de Communication et Adaptation des Micro-organismes |
Name Institute | Muséum National d'Histoire Naturelle, Sorbonne Universités |
City | Paris |
Country | France |
top of page | |
Name of the mutant parasite | |
RMgm number | RMgm-4682 |
Principal name | ΔPbkin8B |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
top of page | |
Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Normal production of male and female gametocytes. No male gamete formation as shown by absence of exflagellation centers. |
Fertilization and ookinete | Strongly reduced fertilisation and ookinete formation. In wild type parasites, the mean ookinete conversion rate as 63.5 ± 3.6, whereas it was only 1.2 ± 1.4 and 0.2± 0.2 for ΔPbkin8B clones 3600 and 3716. |
Oocyst | Strongly reduced fertilisation and ookinete formation. In wild type parasites, the mean ookinete conversion rate as 63.5 ± 3.6, whereas it was only 1.2 ± 1.4 and 0.2± 0.2 for ΔPbkin8B clones 3600 and 3716. The two ΔPbkin8B mutant cell lines produced very few oocysts (mean range of 1.68/1.36 and 1.4/0.76 respectively) in comparison to wt and Pbkin8B-gfp parasites, where numerous oocysts were formed (mean values the 2 replicates of 180/171 and 187/160 oocysts per midgut respectively. No sporozoite formation observed in oocysts. |
Sporozoite | The two ΔPbkin8B mutant cell lines produced very few oocysts (mean range of 1.68/1.36 and 1.4/0.76 respectively) in comparison to wt and Pbkin8B-gfp parasites, where numerous oocysts were formed (mean values the 2 replicates of 180/171 and 187/160 oocysts per midgut respectively. No sporozoite formation observed in oocysts. |
Liver stage | No infection of mice by bite of infected mosquitoes |
Additional remarks phenotype | Mutant/mutation Evidence is presented that: |
top of page | |||||||||||||||||||||||||
Details of the target gene | |||||||||||||||||||||||||
Gene Model of Rodent Parasite | PBANKA_0202700 | ||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_0111000 | ||||||||||||||||||||||||
Gene product | kinesin-8B, putative | ||||||||||||||||||||||||
Gene product: Alternative name | |||||||||||||||||||||||||
top of page | |||||||||||||||||||||||||
Details of the genetic modification | |||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||
Type of plasmid/construct used | (Linear) plasmid double cross-over | ||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | Yes | ||||||||||||||||||||||||
Name of PlasmoGEM construct/vector | PbGEM-267699 | ||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||
Partial or complete disruption of the gene | Complete | ||||||||||||||||||||||||
Additional remarks partial/complete disruption | |||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | hdhfr/yfcu | ||||||||||||||||||||||||
Promoter of the selectable marker | eef1a | ||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||
Additional remarks genetic modification | |||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||
Primer information: Primers used for amplification of the target sequences
![]() Primer information: Primers used for amplification of the target sequences
![]()
| |||||||||||||||||||||||||
top of page |