| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption,
Introduction of a transgene,
Introduction of a transgene
|
| Reference (PubMed-PMID number) |
|
| MR4 number |
37029167
|
| top of page |
| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
RMgm-1320
|
| Other information parent line | This transgenic reporter line (1868cl1) expresses luciferase under the control of the eef1α (PBANKA_1133300) promoter and, in addition, mCherry under the control of the hsp70 (PBANKA_0711900) promoter. Both reporter cassetes are introduced into the silent 230p locus, using a single DNA construct. This transgenic line does not contain a drug-selectable marker |
| top of page |
| The mutant parasite was generated by |
| Name PI/Researcher | Franke-Fayard B, Janse CJ |
| Name Group/Department | Leiden Malaria Research Group, Parasitology |
| Name Institute | Leiden University Medical Center (LUMC) |
| City | Leiden |
| Country | The Netherlands |
| top of page |
| Name of the mutant parasite |
| RMgm number | RMgm-4681 |
| Principal name | 3065cl1 |
| Alternative name | PbANKA-CS GIMO (mCherry-Luc) |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
| top of page |
| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Normal numbers of oocysts are produced in Anopheles stephensi mosquitoes. Sporozoite formation within the oocysts is profoundly inhibited: up to day ten after feeding of the mosquitoes the morphology of oocysts is normal; after day 10, the oocysts display a highly vacuolated structure. No sporozoite formation. |
| Sporozoite | No sporozoite formation in oocysts; No salivary gland sporozoites |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of CSP. In the mutant the endogenous P. berghei csp gene has deleted by replacing the csp gene with the drug-selectable marker cassette hdhfr-yfcu. It also expresses mCherry and luciferase under constitutive promoters
Protein (function)
The CS protein is the major protein on the surface of sporozoites and is critical for development of sporozoites within the oocysts and is involved in motility and invasion of both the salivary gland of the mosquito and the liver cells. The protein is also found on the oocyst plasma membrane and on the inner surface of the oocyst capsule. Specific motifs in CS are involved in sporozoite binding to mosquito salivary glands and in sporozoite attachment to heparan sulfate proteoglycans in the liver of the mammalian host. During substrate-dependent locomotion of sporozoites, CS is secreted at the sporozoite anterior pole, translocated along the sporozoite axis and released on the substrate at the sporozoite posterior pole. Following sporozoite invasion of hepatocytes, the CS is released in the host cell cytoplasm.
Phenotype
Normal numbers of oocysts are produced in Anopheles stephensi mosquitoes. Sporozoite formation within the oocysts is profoundly inhibited: up to day ten after feeding of the mosquitoes the morphology of oocysts is normal; after day 10, the oocysts display a highly vacuolated structure. No sporozoite formation. No sporozoite formation in oocysts; No salivary gland sporozoites
Additional information
Other mutants |
*RMgm-4681
