RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-4671
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_0942700; Gene model (P.falciparum): PF3D7_1104200; Gene product: chromatin remodeling protein (SNF2L)
Name tag: triple-HA
Phenotype Asexual bloodstage;
Last modified: 18 August 2019, 13:57
  *RMgm-4671
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 31390575
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
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The mutant parasite was generated by
Name PI/ResearcherHillier C, Billker O, Choudhary JS
Name Group/DepartmentFunctional Proteomics
Name InstituteThe Institute of Cancer Research
CityLondon
CountryUK
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Name of the mutant parasite
RMgm numberRMgm-4671
Principal namePBANKA_0942700-3xHA
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
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Phenotype
Asexual blood stageExpression in schizonts. Epitope-tagged PBANKA_0942700 was immunoprecipitated from the lysates of P. berghei schizonts, and MS identification of co-purifying proteins confirmed interaction with different proteins.
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant expresses a C-terminal 3xHA-tagged version of PBANKA_0942700

Protein (function)
A putative chromatin remodeling protein

Phenotype
The protein was identified in a large-scale protein interaction network analysis. Affinity purification followed by MS analysis of HA-tagged SNF2L, a putative chromatin remodeling protein, identified uncharacterized protein PBANKA_1461600, confirming a physical link between these 2 proteins, which are connected in the network through the eIF3 complex

Additional information

Other mutants

  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_0942700
Gene Model P. falciparum ortholog PF3D7_1104200
Gene productchromatin remodeling protein
Gene product: Alternative nameSNF2L
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Details of the genetic modification
Name of the tagtriple-HA
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedYes
Name of PlasmoGEM construct/vectorPbGEM-292320
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren