Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene disruption
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Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 31202684 |
MR4 number |
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Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
P. berghei ANKA 2.34
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Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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The mutant parasite was generated by |
Name PI/Researcher | Zhu X, Cui L |
Name Group/Department | Department of Immunology, College of Basic Medical Science |
Name Institute | China Medical University |
City | Shenyang |
Country | China |
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Name of the mutant parasite |
RMgm number | RMgm-4640 |
Principal name | Δpbpp5 |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Normal (wild type) numbers of male and female gametocytes produced. Strong reduction in male gamete formation (exflagellation): 13.8 – 25% exflagellating Δpbpp5 male gametocytes compared with 88.9% in wild type parasites. |
Fertilization and ookinete | No ookinete formation in vitro |
Oocyst | No ookinete formation in vitro. Strong reducuction of oocyst formation (70-100%). |
Sporozoite | No ookinete formation in vitro. Strong reducuction of oocyst formation (70-100%). No sporozoites detected in salivary glands |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of PP5
Protein (function)
Malaria parasites have homologues of all major human phosphoprotein phosphatase (PPP) subfamilies PP1 – PP7. Of the PPP subfamilies, PP5 differs from other phosphatases in possessing three N-terminal tetratricopeptide repeat (TPR) domains, which are auto-inhibited and responsible for protein-protein interactions. Being expressed in virtually all mammalian systems, PP5 interacts with a number of proteins through its TPR domains to function as a modulator in multiple signaling pathways.
Phenotype
Normal (wild type) numbers of male and female gametocytes produced. Strong reduction in male gamete formation (exflagellation): 13.8 – 25% exflagellating Δpbpp5 male gametocytes compared with 88.9% in wild type parasites.
No ookinete formation in vitro. Strong reducuction of oocyst formation (70-100%).
No sporozoites detected in salivary glands.
Crossing experiments showed that female Δpbpp5 gametocytes/gametes are fertile whereas Δpbpp5 male gametocyte/gamete fertility is strongly reduced.
Additional information
See RMgm-4641 for a mutant expressing C-terminal GFP-tagged version PP5: the PbPP5 protein was expressed in both blood stages and ookinetes with more prominent expression during sexual development. PbPP5 was localized in the cytoplasm of the parasite and highly concentrated beneath the parasite plasma membrane in free merozoites and ookinetes.
Other mutants |