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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1224400
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Gene Model P. falciparum ortholog |
PF3D7_0806300
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Gene product | ferlin, putative |
Gene product: Alternative name | ferlin-like protein, FLP |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct used | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Partial or complete disruption of the gene | Complete |
Additional remarks partial/complete disruption |
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | From the paper;
'Attempts to delete flp using standard gene targeting approaches with the plasmid b3D.DT∧H.∧D or PlasmoGEM vectors however failed repeatedly in our hands, and a recent global screen identified the gene to be essential for the blood stage. This strongly suggests that FLP fulfils an essential function during the intra-erythrocytic development cycle.
The P. berghei ferlin-like protein (FLP, PBANKA_1224400) contains six predicted C2 domains, a C-terminal transmembrane domain and a Fer domain (a ferlin-specific motif of unknown function). These typical features cluster FLP together with ferlin (PBANKA_1319300) into the ferlin protein family.Ferlins are typically embedded in vesicular membranes via a single, C-terminal transmembrane domain with the N-terminus of the protein facing the cytosol.' |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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