RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-4562
Malaria parasiteP. berghei
Genotype
MutatedGene model (rodent): PBANKA_0403200; Gene model (P.falciparum): PF3D7_0304600; Gene product: circumsporozoite (CS) protein (CSP)
Details mutation: The P. berghei csp gene replaced by P. falciparum csp
Transgene
Transgene not Plasmodium: mCherry
Promoter: Gene model: PBANKA_0501200; Gene model (P.falciparum): PF3D7_1016900; Gene product: early transcribed membrane protein 10.3 | protein of early gametocyte 4 (UIS4, up-regulated in infective sporozoites; ETRAMP10.3)
3'UTR: Gene model: PBANKA_0501200; Gene product: early transcribed membrane protein up-regulated in infective sporozoites (UIS4, up-regulated in infective sporozoites; ETRAMP10.3)
Replacement locus: Gene model: PBANKA_0306000; Gene product: 6-cysteine protein (230p)
PhenotypeNo phenotype has been described
Last modified: 30 December 2018, 21:48
  *RMgm-4562
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene mutation, Introduction of a transgene
Reference (PubMed-PMID number) Reference 1 (PMID number) : 26271010
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone RMgm-1339
Other information parent lineThis mutant expresses mCherry under control of the UIS4 promoter. mCherry expression in sporozoites and in liver stages.
The mutant parasite was generated by
Name PI/ResearcherFlores-Garcia Y, Zavala F, Sinnis P
Name Group/DepartmentDepartment of Molecular Microbiology & Immunology
Name InstituteJohns Hopkins Bloomberg School of Public Health
CityBaltimore
CountryUSA
Name of the mutant parasite
RMgm numberRMgm-4562
Principal namePbPfCSP
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation
In the mutant the P. berghei csp gene has been replaced with the P. falciparum csp gene (using the construct pR-CSPfFL containing the P. falciparum csp coding region with a P. berghei signal sequence, the hDHFR selection cassette, and csp 5'and 3'untranslated regions (UTRs); see RMgm-4563). In addition it expresses mCherry under control of the UIS4 promoter (mCherry expression in sporozoites and liver stages).

Protein (function)
The CS protein is the major protein on the surface of sporozoites and is critical for development of sporozoites within the oocysts and is involved in motility and invasion of both the salivary gland of the mosquito and the liver cells. The protein is also found on the oocyst plasma membrane and on the inner surface of the oocyst capsule. Specific motifs in CS are involved in sporozoite binding to mosquito salivary glands and in sporozoite attachment to heparan sulfate proteoglycans in the liver of the mammalian host. During substrate-dependent locomotion of sporozoites, CS is secreted at the sporozoite anterior pole, translocated along the sporozoite axis and released on the substrate at the sporozoite posterior pole. Following sporozoite invasion of hepatocytes, the CS is released in the host cell cytoplasm.

Phenotype
From the paper:

'To determine if the antibody response that impacted sporozoite motility after immunization with irradiated sporozoites was specific to CSP, we generated a fluorescent P. berghei parasite in which the endogenous csp gene was replaced by the csp coding sequence from the human malaria parasite Plasmodium falciparum (PbPfCSP). Importantly, antibodies to P. falciparum CSP do not cross-react with P. berghei CSP, and vice versa. Transgenic PbPfCSP parasites develop normally in the mosquito and display normal infectivity in mice.
To look at the role of CSP-specific antibodies on sporozoite motility in the skin, mice were immunized with P. berghei sporozoites, and following immunization, the motility of PbPfCSP sporozoites in immunized and naive mice was assessed by confocal microscopy. In contrast to the significant differences in net displacement and average speed of wild-type P. berghei sporozoites, we found no significant difference in either parameter when PbPfCSP mCherry parasites were inoculated into P. berghei-immunized mice. In contrast, when mice were immunized with PbPfCSP parasites, net displacement and speed of PbPfCSP sporozoites were significantly decreased. Taken together, sporozoite immunization impacts the motility of sporozoites at the inoculation site and appears to be mostly directed against the CS protein of sporozoites.'

Additional information


Other mutants


  Mutated: Mutant parasite with a mutated gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0403200
Gene Model P. falciparum ortholog PF3D7_0304600
Gene productcircumsporozoite (CS) protein
Gene product: Alternative nameCSP
Details of the genetic modification
Short description of the mutationThe P. berghei csp gene replaced by P. falciparum csp
Inducable system usedNo
Short description of the conditional mutagenesisNot available
Additional remarks inducable system
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markernot available
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationIn the mutant the P. berghei csp gene has been replaced with the P. falciparum csp gene (using the construct pR-CSPfFL containing the P. falciparum csp coding region with a P. berghei signal sequence, the hDHFR selection cassette, and csp 5'and 3'untranslated regions (UTRs)). See RMgm-4563
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6

  Transgene: Mutant parasite expressing a transgene
Type and details of transgene
Is the transgene Plasmodium derived Transgene: not Plasmodium
Transgene namemCherry
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Click to view information
Click to hide information
Plasmid/construct sequence
Click to view information
Click to hide information
GGCCGCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGC
TTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGC
GAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCC
CTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTG
AAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGG
GAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG
CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGC
CCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAG
GACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTAC
GACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTAC
AACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAG
TACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAATCT
GATATCTATAATTCATTATGAGTAGTGTAATTCAGAAAGAGAACAAATTGTTTTATGATA
AATATATTTATAAAATAAAAAGTAAGAGAAAAGGAAAAATAATGTTATAGAGAAAAAAAG
GGAAAACGTTATAAAAAAATAGCATCTTAACTATTATTATTTTACTTTATGTGTGTATAA
AACTTTTACAATATATTTTTTTATTTTGAACGTATATAAAAATATTTCCTTTAATTTGTA
GTGTGCAAATAATTTATAAAAATAATTTTTTTAAATATAAATATATTGTCTCGAAAAGAA
ATGAAAATGGATTCTAGCAAATAAACAAGTCTGGCTTCGAAAAGAAATATAAGGCAAAAA
TTATATATAATATATTTGAAAATTATATTTGTGAGAGTGTGTATTATTTTTGTTTGAAAG
ATAATTTTATGGGTTTTTGAGAGTGATTTTTAGTGTTTTATTTGTTTTTTTTTAATAATT
TGTAAGTTTTGTCAATGTAGAGAGAATTATACTTCATAACGTATTATTTGAACGATTAAA
AAATATTTTTTTTAAACAGTGAAATATAAATATGAATGGAAGCAGCCGTATATGCAAACT
TTTTTACATATGTTGTATGCATAACATGCGTTTTTATTTTTTACGAAGTATACATAATTC
CCTTTAAAATATAACTTTATATAATTTGTTTTTATTGTAATGGATATAGTATTGTATAAA
TTTTATATTTATTTCCTAAAGACTTTTTCATAAAAATTGTTGGTGTTCCAGATATATTAT
TTTTAAAAGTCAATATATCAAGTTGTTATATTGTCTTAGAGTTCAAAAGTTTGTCCTAAT
TGAATTTTTTATAACTTTATAATATTTATTGTTTTACTTTGGACCATTAAGTATATATAA
GTAGTAACTTATTAAAATAATAAATTATATGTATCATAATTCATGGCTTTATAGATTCTG
CATTAACCTTAAATATGAAAAACACAAAAATGATGACAAGCATTAAAGCGAAAGGTACCG
AGCTCGAATTCTCTTGAGCCCGTTAATGAAATAGATACAATTCATTCATGTTATATACAT
CTAGAACATAATCTGAATATGGTTCAAGTTAAATGTCCAAAAATTATAAAAAGTGATGAT
ATTTTTGATGGTAATACCATAATAGACACCAAGGTAACATCACGAAGTAGTCAACAAAAT
AATTTTTATTTAGAAAATACAGATGTTGAACCAGAAGAAATAGAGAAATATAAAAATATA
GAATACATACCAGAAAACGATGAAGTAATGCATCTAGACAAAAAAGAAAAGCTAGATGAT
ATATTACCAGGTGTTATCATATTAGATAAAAATAAAATGTTCAAAGAAAAAGGACATTTC
ACTTTTGTTACTCCATTAATTGTAGAAAAGGTATTAATATTAAAAATATATTGTGATAAT
ACTAAAACAATAATTAATAATATGAAAGGGAAAAAAGGTATTACAGTAATAAGGATTTCT
CAAAATACAACAAAAAATAAATTTTATGGATGTGACTTTTCAGGTAATTCTAAAAAAACA
TTTTACTATTCCAATGTTTATGATTTAGAAAAAAAAAATGAGTTTTGTGAAATAGAATTA
AAAGAAAATATAGTAGTTAGCTTAAATTGTCCAACTGGTAAAATTAATCCAAAAAATTGT
TTTAGAAATGTATATATAAAAAGTAATATGAATGAACAAACAACCGAAAATATAGAAAAT
ATATTTAACGAAATAAAAGTTATAGATGCAGATTATTTTATAAATAATTCATCAACCTTT
TTGATGATTTCCAAAATTACAAAAAAAGAGTTTGATTTTTATTGTACATGTGAAGATTAT
AAAACCAAAAATATAGGAACAATATATATTAAAAATTATGAATATCTAGATTCAAAACCT
AAATATAAAAATAAACAAATTTCCTATATAGATGTAGTTCCATACCCGCGGGGAAAGGGC
GAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACT
TAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAC
CGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTT
TCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTG
CTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTG
ACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTG
CATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGAT
ACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCAC
TTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATAT
GTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG
TATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCC
TGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGC
ACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCC
CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATC
CCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTT
GGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATT
ATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGAT
CGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCT
TGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGAT
GCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGC
TTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG
CTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTC
TCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTA
CACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGC
CTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA
TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCAT
GACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGAT
CAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA
ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAA
GGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTT
AGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTT
ACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATA
GTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTT
GGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCAC
GCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGA
GCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCG
CCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAA
AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACAT
GTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGC
TGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGA
AGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTG
GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTA
GCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG
AATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCT
TCCGCGGGTATATGGTAAAGAACCTACTAACACAATAAAATATTTAAATAATGTATTTCC
TATAAATAAATTTACAGATTTATTTTTTAATACAAAAGATATAGATATACCAGAAATAAA
TGATCAGTTTAAAGGTTTTAAATTCTTTATGACATCATTTATAAATCATGGATCATATCC
ACTAACAATAGAATGTGGTGTAACAAATGGTGGAACTAGTTATAAAAGAGCAATTATTTT
ATTGCATGTTCGAACTGATTTAAAAGATAGACCAGTTTCATTTTGTGATTTTCGAAAAGG
AGAATTATATAATTATTTGAATGCTTATACTGAAGGGGATGTATGCATAATAATTTCCAA
ATCAAATACAAGTTTTGGTTTTAGATGCCCAGTAAATACAAAAAAAATGCCAAAAAATTG
TTTTACGCAAGTATATGAAAAAGGGTATCTAAATGACGCCAATAAAATTAATACTAAAAA
TGTTATTAACTATTCATTTGAAAATCCAGAATATGCGCTAGCTGGTTYTAATTATACATT
AACAAAATCGTATCAATTTGAATGTCATTGTGTAGATAAAGAAACAGAACAAATTGTAAA
AACGGTTTTAGTCAAATATGTAAATGAAGATGAAATATATGATTATAATGATTTTCCAAT
GGTGAATCACAAACCTATTATTGCACATCCAAATAAAACACATCAAGCTTGCATGCCTGC
AGGGTGATAGTGTAGATTTTTTTGTTTGACCATTGCATCATTGTCTTTTATTCATCGATT
TTATTATGTTATATATATTTTTTTAAATATCATAATATTTTTTCAATATAGCTTCTTTGA
GCAAATACTGAACAATAAGGAATGCTTCTATGCATCCGTGGATGTATATGTACGTAATAA
AATAATTTTTTGTCCATAAAAATATCTTTAACAGCTGTTATACAAAAGCATTGAATTGGC
TGATACATTTTTGTCGTAAATAAAATAAATGAATTAATGGTACAAAATTACAATAAAAAG
GAAGCGAATTTATTGCATTTTGTTTAATTTATGTCCTTTCCATTTTATTAATACAATTAT
GGTACTAGTAATTCGTATTAAACATATATTTTGCATGTACCAAATGTTTGGGAAATAAGC
ATATGGATCCCACTAAATTTAATAATATTCTGCGATTTTTCTTATATTTACTATTAAATA
TAATGGATTCATTTTTTGATGCATGCAATTTTTTCTTTTAATGTATTAATTAGTGTGACA
ATTTTGAAAAACTTTACTCATTTATTTATTTTCCTTATTATATTGGATTTATAATACCAG
TATTATTATATAAACCACATTAAATAATTTGTAATTTATTCGAGGGTATCAAAAAAAACA
TATACAAAATGCATATATCCACATATGGCTCATTATAGGGTGAATAAAAATGGGAACAGC
AGATTAATTATTATATCATGAAAGTAATGAAAAAATTTAAATTATATGGATATATACATA
TATAATATGTTAAAAAATAATAATAAATAAATAAAATGTATTATAAATCTTATAAAATAA
GTTTTGTTGTGAATAATCCACGAAATATGCCATAAATAGACACTGAACAAATTAGTGGTT
CTTAATATTATTTTGGATACATGCGGATATTATCATTGTCAGATGATTTATTTTTTGTTA
TTTTTAAATTATAAATATTCATATTTTATAGTCCTCAAAAAATAGGATGTTTTATTTTTT
TATAGCTATATTTTATGGTTGATCCTTTCCTTTTATGGTGTTTCATAAAAATTTTATTGA
GCTATATACAATCCAATAAAAAAAGGCAATTGAATTTTGAAATATATTAAACTATTTTTA
TAATAAAATAAATATAATTTTTAAATAATGTTATATTATATATAATTTTTATCTTTACAC
AGAAATTTTTTTTTAGAGTCCAATATATATAATTAGTTATATATATAGAGATATACACTA
CATACACCACCATAAATAATTATAAGGAAAACCAGTTATTTTAATTTTAACTGAAGAAAT
TAAATAAATATATAAAAAAGAAAAGAACAAAAAATAAAACGACAGCAACCTTAAAATTTT
ATTATTATTACATATTTATACATAAAAAAAATAAAATACTTAGACAAAAAATAATAAATA
TTATAGATCGATATTAGCATACATATATACCTTTCAGCACATAATTATTACGTCTGC
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedureNo
Selection (negative) procedure5-fluorocytosine (5-FC)
Additional remarks genetic modificationDNA constructs for generation of the transgenic reporter line expressing mCherry is based on the standard GIMO DNA construct pL0043 (Lin, Annoura et al. 2011). This construct contains the 5’ and 3’ targeting sequences (TRs) for the 230p locus as well as a multiple-cloning site for integration of transgene-expression cassettes. This construct integrates by double cross-over homologous recombination and replaces the positive-negative selectable marker (hdhfr::yfcu; SM) cassette with the transgene-expression cassette in the 230p locus in P. berghei parasites of GIMOPbANKA. The mCherry expression cassette contains mCherry flanked by the 5’ and 3’UTR (promoter and transcription terminator) sequences of P. berghei uis4 (PBANKA_050120) which were amplified from P. berghei WT genomic DNA. The 5'UTR was amplified with primer pair 7169 (tatcctgcaggGTGATAGTGTAGATTTTTTTGTTTGAC) and 7170 (ataagaatgcggccgcAGACGTAATAATTATGTGCTGAAAGG). The 3'UTR was amplified with primer pair 7171 (cggatatcTATAATTCATTATGAGTAGTGTAATTCAG) and 7172 (ggccggtaccTTTCGCTTTAATGCTTGTCATC)

The mCherry-expression cassette was cloned in pL0043 resulting in plasmid pL1937.
Additional remarks selection procedureThis reporter mutant expressing mCherry does not contain a drug-selectable marker.
The mutant has been generated in the reference line GIMOPbANKA (RMgm-687). The GIMO mother line is used for introduction of transgenes into the modified 230p locus through transfection with constructs that target the 230p locus. These constructs insert into the 230p locus (‘gene insertion’), thereby removing the hdhfr::yfcu selectable marker (‘marker out’) from the genome of the mother lines. Transgenic parasites that are marker-free are subsequently selected by applying negative drug selection using 5-FC. This selection procedure is performed in vivo in mice.
Other details transgene
Promoter
Gene Model of Parasite PBANKA_0501200
Gene Model P. falciparum ortholog PF3D7_1016900
Gene productearly transcribed membrane protein 10.3 | protein of early gametocyte 4
Gene product: Alternative nameUIS4, up-regulated in infective sporozoites; ETRAMP10.3
Primer information details of the primers used for amplification of the promoter sequence  Click to view information
Primer information details of the primers used for amplification of the promoter sequence  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
3'-UTR
Gene Model of Parasite PBANKA_0501200
Gene productearly transcribed membrane protein up-regulated in infective sporozoites
Gene product: Alternative nameUIS4, up-regulated in infective sporozoites; ETRAMP10.3
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to view information
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Insertion/Replacement locus
Replacement / InsertionReplacement locus
Gene Model of Parasite PBANKA_0306000
Gene product6-cysteine protein
Gene product: Alternative name230p
Primer information details of the primers used for amplification of the target sequences  Click to view information
Primer information details of the primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4