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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_0807100
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| Gene Model P. falciparum ortholog |
PF3D7_0318100
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| Gene product | stomatin-like protein |
| Gene product: Alternative name | STOML |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
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| Type of plasmid/construct used | (Linear) plasmid double cross-over |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
|
| Plasmid/construct map |
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| Plasmid/construct sequence |
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| Restriction sites to linearize plasmid |
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| Partial or complete disruption of the gene | Complete |
| Additional remarks partial/complete disruption |
|
| Selectable marker used to select the mutant parasite | hdhfr/yfcu |
| Promoter of the selectable marker | pbdhfr |
| Selection (positive) procedure | pyrimethamine |
| Selection (negative) procedure | No |
| Additional remarks genetic modification | The unsuccessful attempts to disrupt this gene indicate an essential function during asexual blood stage growth (see also RMgm-2325 for evidence for successful deletion of this gene)
Proteins of the stomatin/prohibitin/flotillin/HfIK/C (SPFH) family are membrane-anchored and perform diverse cellular functions in different organelles. A systematic database search for Plasmodium SPFH proteins uncovered four candidates that either display a close phylogenetic relationship to conserved members—prohibitin 1 (PHB1, PBANKA_0701500), prohibitin 2 (PHB2, PBANKA_1213100), and stomatin-like protein (STOML, PBANKA_0807100)- 0807100)—or fall into a unique sister group that contains related proteins from the phylum Myzozoa, which harbors the subphylum Apicomplexa. Accordingly, the latter Plasmodium protein was named prohibitin-like protein (PHBL, PBANKA_0718600). All four proteins contain a central signature SPFH domain and a hydrophobic stretch Although the conventional Plasmodium SPFH proteins display a similarly high degree of conservation, PHBL sequences are rather divergent already among related Plasmodium species. |
| Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences  Primer information: Primers used for amplification of the target sequences 
| Sequence Primer 1 | |
| Additional information primer 1 | |
| Sequence Primer 2 | |
| Additional information primer 2 | |
| Sequence Primer 3 | |
| Additional information primer 3 | |
| Sequence Primer 4 | |
| Additional information primer 4 | |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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*RMgm-4445