| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
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| Reference (PubMed-PMID number) |
Not published (yet)
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
P. berghei ANKA cl15cy1
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| Other information parent line | A reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255). |
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| The mutant parasite was generated by |
| Name PI/Researcher | Religa AA, Janse CJ, Waters AP |
| Name Group/Department | Leiden Malaria Research Group |
| Name Institute | Leiden University Medical Center |
| City | Leiden |
| Country | The Netherlands |
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| Name of the mutant parasite |
| RMgm number | RMgm-4440 |
| Principal name | 503cl1 |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Evidence for PbSIR2A::GFP in asexual blood stages (mainly nuclear location). |
| Gametocyte/Gamete | Evidence for PbSIR2A::GFP in cytoplasm of gametocytes (higher in females than in males). |
| Fertilization and ookinete | Evidence for PbSIR2A-GFP signal in mauture ookinetes concentrated peripherally near the apical end as well as the nucleus borders (circle-like staining). The characteristic apical-proximal localisation starts forming between 6 - 9h post-fertilisation and is most prominent in mature ookinetes, but is not present in young ookinetes (<6hrs post-fertilisation). |
| Oocyst | Evidence for PbSIR2A::GFP in (cytoplasm of) oocysts. |
| Sporozoite | Not tested |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of SIR2a
Protein (function)
Phenotype
Evidence for PbSIR2A::GFP in asexual blood stages (mainly nuclear location). Evidence for PbSIR2A::GFP in cytoplasm of gametocytes (higher in females than in males). Evidence for PbSIR2A-GFP signal in mature ookinetes concentrated peripherally near the apical end as well as the nucleus borders (circle-like staining). The characteristic apical-proximal localisation starts forming between 6 - 9h post-fertilisation and is most prominent in mature ookinetes, but is not present in young ookinetes (<6hrs post-fertilisation). Evidence for PbSIR2A::GFP in (cytoplasm of) oocysts.
See also mutant RMgm-4435 that lacks expression of SIR2a: Evidence is found that ookinetes do not traverse the midgut epithelium. No oocyst formation. No sporozoite formation.
Additional information
Other mutants
- see also mutant RMgm-4435 that lacks expression of SIR2a
- a mutant lacking sir2b - RMgm-4436
- a double gene knockout lacking both sir2b and sir2a (see mutant RMgm-4437).
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*RMgm-4440
StudioDrie; S. Mededovic and A. van Wigcheren