Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene disruption
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Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 29579782 |
MR4 number |
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Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
Not applicable
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Other information parent line | |
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The mutant parasite was generated by |
Name PI/Researcher | Hallée S, Mair GR, Richard D |
Name Group/Department | Centre de recherche en infectiologie |
Name Institute | CHU de Québec-Université Laval |
City | Quebec City, Quebec |
Country | Canada |
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Name of the mutant parasite |
RMgm number | RMgm-4439 |
Principal name | Δgp2 |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Not different from wild type |
Sporozoite | Not different from wild type |
Liver stage | Not different from wild type |
Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of GP2
Protein (function)
Eveidence is provided that GP2 (Golgi protein 2) forms a complex with Golgi protein 1 (PF3D7_1320000; rhoptry protein 2, putative; golgi protein 1; PBANKA_1418300). The Golgi Protein complex localizes to the cis-Golgi throughout the erythrocytic cycle and potentially also during the mosquito stages.
Phenotype
GP2 is not essential throughout the complete lifecycle. No phenotypes were detected different from wildtype.
Additional information
The extensive colocalization between GP2 and the cis-Golgi apparatus marker Endoplasmic reticulum Retention Defective 2 (ERD2) confirmed that GP2 resides in the Golgi throughout the blood stages. This staining pattern was conserved in the rodent malaria parasite P. berghei, where we tagged the endogenous locus of the orthologous gene PBANKA_092480 with GFP. The tagged protein was also detected in gametocytes, as well as all mosquito stage parasites including the ookinete, midgut oocysts, and sporozoites providing evidence for the constitutive expression of GP2 throughout the life cycle of the parasite. In addition, the staining pattern of PbGP2 in the mosquito stages resembled what was previously described with PbGP1 which suggests that they might perhaps also form a complex in this phase of the life cycle
Other mutants
4438: a mutant expressing a C-terminal GFP-tagged version of GP2 |