Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene tagging
|
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 29579782 |
MR4 number |
|
top of page |
Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
Not applicable
|
Other information parent line | |
top of page |
The mutant parasite was generated by |
Name PI/Researcher | Hallée S, Mair GR, Richard D |
Name Group/Department | Centre de recherche en infectiologie |
Name Institute | CHU de Québec-Université Laval |
City | Quebec City, Quebec |
Country | Canada |
top of page |
Name of the mutant parasite |
RMgm number | RMgm-4438 |
Principal name | gp2::gfp |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
top of page |
Phenotype |
Asexual blood stage | expression in blood stages |
Gametocyte/Gamete | expression in gametocytes |
Fertilization and ookinete | expression in ookinetes |
Oocyst | expression in oocysts |
Sporozoite | Not tested |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of GP2
Protein (function)
Eveidence is provided that GP2 (Golgi protein 2) forms a complex with Golgi protein 1 (PF3D7_1320000; rhoptry protein 2, putative; golgi protein 1; PBANKA_1418300). The Golgi Protein complex localizes to the cis-Golgi throughout the erythrocytic cycle and potentially also during the mosquito stages.
Phenotype
Expression of GFPtagged GP2 in multiple blood and mosquito stages
Additional information
The extensive colocalization between GP2 and the cis-Golgi apparatus marker Endoplasmic reticulum Retention Defective 2 (ERD2) confirmed that GP2 resides in the Golgi throughout the blood stages. This staining pattern was conserved in the rodent malaria parasite P. berghei, where we tagged the endogenous locus of the orthologous gene PBANKA_092480 with GFP. The tagged protein was also detected in gametocytes, as well as all mosquito stage parasites including the ookinete, midgut oocysts, and sporozoites providing evidence for the constitutive expression of GP2 throughout the life cycle of the parasite. In addition, the staining pattern of PbGP2 in the mosquito stages resembled what was previously described with PbGP1 which suggests that they might perhaps also form a complex in this phase of the life cycle
Other mutants
RMgm-4439: a mutant lacking GP2 |