Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of PhIL1

Protein (function)
PhIL1 is part of the inner membrane complex (IMC)

Phenotype
Unsuccessful attempts to disrupt this gene indicate an essential function during asexual blood stage growth (see RMgm-4366).
PhIL1-GFP expression in early/late schizonts (blood and liver), in ookinetes and sporozoites

Additional information
In the paper evidence is presented that:
i) PhIL1 is part of the inner membrane complex (IMC). See also RMgm-4368)
ii) PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages.

To investigate further the location of PhIL1 relative to other apical and uniform IMC markers, PhIL1-GFP parasites were crossed with lines expressing ISP1-Cherry (uniform IMC marker) and MyoA-Cherry (apical marker) lines. During liver stage development, PhIL1-GFP co-localized with MyoA-mCherry showing a similar overall distribution. In ookinetes, while a substantial portion of the peripheral weak PhIL1 staining colocalised with ISP1, the very apical end of the ISP1 staining was devoid of PhIL1 staining. MyoA-Cherry also displays a subapical ring-shaped location, but this also appeared as slightly apical to the strong sub-apical staining shown by PhIL1. Therefore PhIL1 displays a distribution in ookinetes unlike that of either ISP1 or MyoA.

Other mutants