Mutant/mutation
The mutant expresses a C-terminal triple Myc tagged version of UIS3. In this study an existing mutant lacking the uis3 gene has been complemented with a triple Myc tagged version of uis3.
The gene has been introduced by a single cross-over construct containing the hdhfr gene (selection with the drug WR99210)

Protein (function)
UIS3 is a protein of the parasitophorous vacuole membrane of liver stages

Phenotype
uis3-myc expression in liver stages. Immunofluorescence analysis of liver-stage schizonts revealed that UIS3-myc co-localized with host LC3 and UIS4 to the PVM.

Additional information
In this paper evidence is presented that
i) Plasmodium berghei parasites infecting hepatic cells rely on the PVM transmembrane protein UIS3 to avoid elimination by host-cell-mediated autophagy.
ii) UIS3 binds host LC3 through a non-canonical interaction with a specialized surface on LC3 where host proteins with essential functions during autophagy also bind.
iii) UIS3 acts as a bona fide autophagy inhibitor by competing with host LC3-interacting proteins for LC3 binding.

Other mutants
See the link PF3D7_1302200