RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-4126
Malaria parasiteP. berghei
Genotype
Genetic modification not successful
MutatedGene model (rodent): PBANKA_0926700; Gene model (P.falciparum): PF3D7_1121600; Gene product: exported protein 1 | circumsporozoite-related antigen | parasitophorous vacuole membrane antigen QF 116 (EXP1; CRA; Ag5.1; HEP17)
Details mutation: Replacement of the promoter region by the ama1-PBANKA_091500-promoter by 'promoter swap' technology
PhenotypeNo phenotype has been described
Last modified: 5 February 2017, 11:09
  *RMgm-4126
Successful modificationThe gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted Gene mutation
Number of attempts to introduce the genetic modification Unknown
Reference (PubMed-PMID number) Reference 1 (PMID number) : 28115054
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
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Attempts to generate the mutant parasite were performed by
Name PI/ResearcherKlug D, Frischknecht F
Name Group/DepartmentIntegrative Parasitology
Name InstituteCenter for Infectious Diseases, Heidelberg University Medical School
CityHeidelberg
CountryGermany
  Mutated: Mutant parasite with a mutated gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_0926700
Gene Model P. falciparum ortholog PF3D7_1121600
Gene productexported protein 1 | circumsporozoite-related antigen | parasitophorous vacuole membrane antigen QF 116
Gene product: Alternative nameEXP1; CRA; Ag5.1; HEP17
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Details of the genetic modification
Short description of the mutationReplacement of the promoter region by the ama1-PBANKA_091500-promoter by 'promoter swap' technology
Inducable system usedNo
Short description of the conditional mutagenesisNot available
Additional remarks inducable system
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationUnsuccessful attempts to disrupt the exp1 indicates an essential function (see RMgm-4125).
Also replacement of the exp1 promoter region with the (schizont-specific) ama1 promoter region were unsuccessful.
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren