RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-4125
Malaria parasiteP. berghei
Genotype
Genetic modification not successful
DisruptedGene model (rodent): PBANKA_0926700; Gene model (P.falciparum): PF3D7_1121600; Gene product: exported protein 1 | circumsporozoite-related antigen | parasitophorous vacuole membrane antigen QF 116 (EXP1; CRA; Ag5.1; HEP17)
PhenotypeNo phenotype has been described
Last modified: 5 February 2017, 11:08
  *RMgm-4125
Successful modificationThe gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted Gene disruption
Number of attempts to introduce the genetic modification Unknown
Reference (PubMed-PMID number) Reference 1 (PMID number) : 28115054
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
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Attempts to generate the mutant parasite were performed by
Name PI/ResearcherKlug D, Frischknecht F
Name Group/DepartmentIntegrative Parasitology
Name InstituteCenter for Infectious Diseases, Heidelberg University Medical School
CityHeidelberg
CountryGermany
  Disrupted: Mutant parasite with a disrupted gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_0926700
Gene Model P. falciparum ortholog PF3D7_1121600
Gene productexported protein 1 | circumsporozoite-related antigen | parasitophorous vacuole membrane antigen QF 116
Gene product: Alternative nameEXP1; CRA; Ag5.1; HEP17
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedYes
Name of PlasmoGEM construct/vector332867
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationThe unsuccessful attempts to disrupt the exp1 indicates an essential function
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren