Mutant/mutation
The mutant lacks expression of AP2-G2 and AP2-G.
To generate the double knock-out mutant the selectable marker from ap2-g ko (RMgm-4101) was removed by negative selection followed by targetting ap2-g2 for deletion.

Protein (function)
Most sequence-specific transcription factor families found in other eukaryotes seem to be absent from Plasmodium. Instead, an expansion of a protein family containing one or more apetala2 (AP2) DNA-binding domains was observed across the phylum apicomplexa. In total, 27 members of this family have been found in the human malaria parasite Plasmodium falciparum (although a possible 28th member of the family may be present. In total, 26 of these have syntenic orthologs in rodent malaria species, each with its unique stage-specific expression profile.
The paper presents a systematic knockout (KO) screen targeting the ApiAP2 family in the rodent malaria parasite P. berghei. Phenotyping of eleven viable ApiAP2 KO mutants reveals ten critical gene functions at different points in the life cycle.

From the paper: To target P. berghei ApiAP2 genes systematically, we succeeded in producing deletion vectors for all but one member of the family (PBANKA_1313200) and transfected each of them into a reporter line constitutively expressing GFP to facilitate phenotyping. Fourteen ApiAP2 genes resisted at least four disruption attempts with up to two different vector designs, providing tentative evidence that more than half of the genes in this family are potentially essential for asexual blood stage growth in vivo. For the remaining eleven, a KO line could be generated. These included six genes that had not previously been studied in Plasmodium.

Phenotype
No gametocyte and ookinete production. See also the phenotypes of ap2-g ko (RMgm-4101) and the ap2-g2 ko (RMgm-4102)
See also the table below.

Based on transcriptome analyses of this mutant and a mutant lacking AP2-G2 (RMgm-4102)  evidence is provided that AP2-G2 is a general repressor of gametocyte-and ookinete-specific genes in (asexual) blood stages

Additional information

From the paper:

Other mutants
See ApiAP2