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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_0306700
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| Gene Model P. falciparum ortholog |
PF3D7_0209600
|
| Gene product | transporter, putative |
| Gene product: Alternative name | TRP |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
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| Type of plasmid/construct used | (Linear) plasmid double cross-over |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
|
| Plasmid/construct map |
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| Plasmid/construct sequence |
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| Restriction sites to linearize plasmid |
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| Partial or complete disruption of the gene | Complete |
| Additional remarks partial/complete disruption |
|
| Selectable marker used to select the mutant parasite | hdhfr/yfcu |
| Promoter of the selectable marker | eef1a |
| Selection (positive) procedure | pyrimethamine |
| Selection (negative) procedure | No |
| Additional remarks genetic modification | The unsuccessful attempts to knock-out this gene indicate an essential role during blood stage development
From the paper: Viable P.berghei mutants lacking the putative lysine decarboxylase/glutamate decarboxylase (ldc- /gad-, PBANKA_100340o) postulated to synthesise GABA from glutamate were generated and grew at the same rate as wild type asexual parasites, indicating that the limited GABA shunt is not essential for asexual stages. Consistent with this conclusion, viable mutant parasite lines lacking the ornithine amino transferase (OAT), a putative GABA/glutamate transaminase which can recycle glutamate, were also obtained (oat-, PBANKA_0107400) and these parasites grew only slightly less quickly than wild type. However, attempts to generate clonal populations of a mutant (trp-, PBANKA_0306700) lacking a putative GABA transporter (TRP) were unsuccessful, suggesting that this transporter may have a broader role than GABA uptake/efflux. Deletion of another amino transferase (PBANKA_0302300) in P.berghei was previously shown to be refractory |
| Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences  Primer information: Primers used for amplification of the target sequences 
| Sequence Primer 1 | |
| Additional information primer 1 | |
| Sequence Primer 2 | |
| Additional information primer 2 | |
| Sequence Primer 3 | |
| Additional information primer 3 | |
| Sequence Primer 4 | |
| Additional information primer 4 | |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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*RMgm-4080