RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-382
Malaria parasiteP. berghei
Genotype
Genetic modification not successful
DisruptedGene model (rodent): PBANKA_1017100; Gene model (P.falciparum): PF3D7_1427500; Gene product: DNA mismatch repair protein MSH2, putative
PhenotypeNo phenotype has been described
Last modified: 24 January 2010, 14:58
  *RMgm-382
Successful modificationThe gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted Gene disruption
Number of attempts to introduce the genetic modification Unknown
Reference (PubMed-PMID number) Reference 1 (PMID number) : 17583362
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei NK65
Name parent line/clone Not applicable
Other information parent line
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Attempts to generate the mutant parasite were performed by
Name PI/ResearcherL. Bethke, D. Wirth
Name Group/DepartmentDepartment of Immunology and Infectious Diseases
Name InstituteHarvard School of Public Health
CityBoston
CountryUSA
  Disrupted: Mutant parasite with a disrupted gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1017100
Gene Model P. falciparum ortholog PF3D7_1427500
Gene productDNA mismatch repair protein MSH2, putative
Gene product: Alternative name
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedunknown
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneUnknown
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasiteunknown
Promoter of the selectable markerunknown
Selection (positive) procedureunknown
Selection (negative) procedureNo
Additional remarks genetic modificationIn this study four genes encoding putative mismatch repair (MMR) proteins have been targeted for disruption in P. berghei. Disruption of the following three genes was unsuccessful: PB300947.00.0 (PF14_0254; MSH2-1; DNA mismatch repair protein Msh2p, putative), PB001297.02.0 (PFE0270c; MSH6; DNA repair protein, putative) and PB000037.02.0 (PF11_0184; MLH1; DNA mismatch repair protein MLH1, putative; mutL homolog). Generation and selection of mutants containing disrupted genes failed for all three genes, suggesting an essential role of these proteins during blood stage development. No information is provided on the (sequence of) DNA constructs used for disruption and on the number of attempts to disrupt these genes.

In the same paper the generation of a mutant lacking expression of PB000623.01.0 (MAL7P1.206; DNA mismatch repair enzyme, putative; MSH2-2) is reported. For this mutant no phenotype has been reported. See RMgm-300.
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren