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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_0831000
|
| Gene Model P. falciparum ortholog |
PF3D7_0930300
|
| Gene product | merozoite surface protein 1 |
| Gene product: Alternative name | MSP-1; MSP1 |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
|
| Type of plasmid/construct used | Unknown |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
|
| Plasmid/construct map |
|
| Plasmid/construct sequence |
|
| Restriction sites to linearize plasmid |
|
| Partial or complete disruption of the gene | Unknown |
| Additional remarks partial/complete disruption |
|
| Selectable marker used to select the mutant parasite | Unknown |
| Promoter of the selectable marker | Unknown |
| Selection (positive) procedure | Unknown |
| Selection (negative) procedure | Unknown |
| Additional remarks genetic modification | In the paper it is reported that repeated attempts were made to disrupt the gene. No information/details is provided on the number of attempts to disrupt the gene, the construct used to disrupt the gene and the sequence of the primers used to amplify the target regions.
A conditional msp-1 knock-out is described in RMgm-270 |
| Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences  Primer information: Primers used for amplification of the target sequences 
| Sequence Primer 1 | |
| Additional information primer 1 | |
| Sequence Primer 2 | |
| Additional information primer 2 | |
| Sequence Primer 3 | |
| Additional information primer 3 | |
| Sequence Primer 4 | |
| Additional information primer 4 | |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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*RMgm-326