Summary

RMgm-301
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_1318600; Gene model (P.falciparum): PF3D7_1454900; Gene product: conserved Plasmodium protein, unknown function
PhenotypeNo phenotype has been described
Last modified: 6 July 2009, 10:34
  *RMgm-301
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Not published (yet)
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255)
The mutant parasite was generated by
Name PI/ResearcherJ.A.M. Braks; C.J. Janse; A.P. Waters
Name Group/DepartmentLeiden Malaria Research Group
Name InstituteLeiden University Medical Center (LUMC)
CityLeiden
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-301
Principal name717cl1
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of PB000633.00.0

Protein (function)
The gene is transcribed in female gametocytes and its transcripts are subject to translational repression in gametocytes (Mair, G.R. et al, 2006, Science 313, 667-669; Braks, J.A.M. et al., 2008, Nucleic acids Research 36, 1176-86).

Phenotype
The phenotype of the mutant has not been analysed in detail. The mutant showed normal growth of the blood stages and normal production of gametocytes and ookinetes. The mutant can be obtained from the Leiden Malaria Research Group.

Additional information

Other mutants


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1318600
Gene Model P. falciparum ortholog PF3D7_1454900
Gene productconserved Plasmodium protein, unknown function
Gene product: Alternative name
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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GTCAACAATAAATAATAAATAAATATTGTGGAAATAAAATAACATATAATTATTTTTAAT
ACATTGATTTCCCTTTTATTTTTTTAAATTTCATTGATATAAAAATATATAATAATAACA
TATATGATTTCAAATTAATCTTTTCAAAAATGGTGTTTATTTTTGTATGTTTGTGTATGA
ATTAATCACATAACACATCTATTAAATTGAGTTGGTAATATAGACACAAATAAATATATA
TATTTTTATAGCTTAAAAGTGTGTTATGAATATTTTAAGCATATTTTCTTTTTCTTTGGA
TTGTGTAAAATGAACTCATATAATGCGTTTTTTTGTTTTTGTTATTTTGTCATTTTGTTA
TTTTGCTATTTTATGGATTAATTTTTGTTTATAAAATGGGAAATAATTTTAACATATTTA
AATAAATGGAGAAAAAATATAAAATAATTATAAAAAAAAGTTAATACACATTTTTTCCTG
TTATAGACCTTATATTTATTTATCCATATATATATATATATATATATATATATATATATA
CATACCAAGTGAATTAAGAGGAAAGCTAATTTATTATTCAGAATAATATATGAACTATAT
ATAATTTTTATTATTTTGGTGTATATTAATCTGTCTATATGCATACATGCAATAATTTAT
CGACTTATATATCAAATAACATAAAATAGAAGTGTTTTAAATTATGGATATATGCTCAAT
ATTCATTTTTTTTAATAAGTTAGCTATATTTAAATTATACATTTTATATATGGTCTCTTT
TTTTTTTAAATATTATTTAAGTGATCATGAAAATATAAATAATTTTTTTTTATTTAATAT
CCTTTTGCTTGCATGTGGTAAATGGAAATTTGGATGTGTTTTGAARGTTCGGATATAGTT
GTATGGACATATAATATATTTTGTGAAAAATTGGTTTTATGTTTATACTTATGCCAATAC
TTTTTGAGTAAAACAAAGCAAGTGCTTATAAATAATTAAAGCCAATTTTATAATATATAT
TTTTTTATTTAATTTGAATTTAGTAGTATAATTTTTTATGGTAAGTGCTCAAAGAGAGTT
GCTTATAAAGTATGGTTTGTTTCTTTTTCGCCATTTTGAATTACACATTAAAAATATATA
GATACATATATTATAATATGAAATCATTAATAATTTAGGGAAATTCTACAAATTTAAAAA
CGAATAAAATAATTGTTTTTCATCATGCCATAACACAATATTGATATATACATGTACAAA
CATTTTTTTTATTTGGAAAATATAAATTATATAAAAAAAAATGTATAGTATACAAAATGA
GCATATTCACACGGGGTGGACGTTCATTTTTTCATTTTTCCCCTGTTTTTTATGAGTATA
TGATAAAATTTTATGAACATTTACACAAAATGAAAATGGATATATAGGAAAAATGGAGCG
GTATTTCATTTATCTTTGATTGTCATTTGGATATTATATTACCYTGGGTAGGCAATTAAA
AATGTTAAATAACAATTTAAGGAAATTATATTTTATATATTAAAATTAACACTGTATTAT
ATGATTCGCTTATAAAAGCCACTCTTTCCCCATGCAAAGCTGTTTAATATCAATTTTAAC
AAATTACACACATGTTAATATATTTATATATATAATTTATATATTTATAATTTATATATT
TATATTTTTATTATTTATATATTTATTATTTATTGTGTGTGTCAATTCGGGTAGGATATA
CCTCTTTTTTATTGTTTAAAGCGATTTGTATTCTAAAATATAAAGRATTTGAAAAAGAGA
AAGATAGAATATGATCCCATCATATATAGCCCTATAATTTTTATTTAGCAGCGAATTAAT
TTTTCTATTAAGTTTATGTGTAATTAAAATAACGGAATATATATAATACAATAAAAAAGT
GCATAAATTAAAATTTTTTCAATTAAATTTTTTTTTTTAAGGGGTTATATAATATTAAAT
ATATAAAATACGATTATATATTTTTGCTACAATTTTTTATATTAAGATATAAATAGTAAA
TAAATGGTATTATATGGCATGTAATATATAAATTTTTTCCAATTTTTATTTTATATACAC
TTTTCCTTTTTTTGTCATAAAACTTAAACAATTTACACATTCATTTTAAAAATTGACTAT
TTGTTTCAACATTTTTTGAGTTTCCGTTTTATAATAGTATTTTCATTTGTATATTGCTTA
TATATATAAATACACACCTAAATGTTACAAAGGATCAATGCATAAACCGGTGTGTCTGGT
CGTCGCGATGACCCCCAAGAGGGGCATCGGCATCAACAACGGCCTCCCGTGGCCCCACTT
GACCACAGATTTCAAACACTTTTCTCGTGTGACAAAAACGACGCCCGAAGAAGCCAGTCG
CCTGAACGGGTGGCTTCCCAGGAAATTTGCAAAGACGGGCGACTCTGGACTTCCCTCTCC
ATCAGTCGGCAAGAGATTCAACGCCGTTGTCATGGGACGGAAAACCTGGGAAAGCATGCC
TCGAAAGTTTAGACCCCTCGTGGACAGATTGAACATCGTCGTTTCCTCTTCCCTCAAAGA
AGAAGACATTGCGGCGGAGAAGCCTCAAGCTGAAGGCCAGCAGCGCGTCCGAGTCTGTGC
TTCACTCCCAGCAGCTCTCAGCCTTCTGGAGGAAGAGTACAAGGATTCTGTCGACCAGAT
TTTTGTCGTGGGAGGAGCGGGACTGTACGAGGCAGCGCTGTCTCTGGGCGTTGCCTCTCA
CCTGTACATCACGCGTGTAGCCCGCGAGTTTCCGTGCGACGTTTTCTTCCCTGCGTTCCC
CGGAGATGACATTCTTTCAAACAAATCAACTGCTGCGCAGGCTGCAGCTCCTGCCGAGTC
TGTGTTCGTTCCCTTTTGTCCGGAGCTCGGAAGAGAGAAGGACAATGAAGCGACGTATCG
ACCCATCTTCATTTCCAAGACCTTCTCAGACAACGGGGTTCCCTACGACTTTGTGGTTCT
CGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCGGAACCGAGCAACGCAATGAGCTC
CTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGGTTGCAGGCTCCTTCTTCGGCCGC
AGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAAGACCGGAAAAAACGCGAGCAAAA
GGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGAGGCCATGAAGAGTTCCAGTACCT
TGATCTCATTGCCGACATTATTAACAATGGAAGGACAATGGATGACCGAACGGGCGTTGG
TGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCGCTGGATCAGGCCTTTCCACTTCT
CACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAAGAGTTGCTGTGGTTCATTCGCGG
CGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTGAAGATCTGGGACAAGAATGTGAC
ACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGAGAGGTCGGAGACATCGGCCCGGG
CTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATACAAAGACATGCACACAGACTACAC
AGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAGATGCTGAGAACGAATCCAACAGA
TCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCGCTGGACGAAATGGCGCTGCCGCC
TTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAGAAGGAGCTGTCGTGCATCATGTA
TCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTCAACATCGCTTCCTATTCGCTTTT
GACGCTCATGGTTGCACACGTCTGCAACCTAAAACCTAAGGAGTTCATTCACTTCATGGG
GAACACGCATGTCTACACGAACCATGTCGAGGCTTTAAAAGAGCAGCTGCGGAGAGAACC
GAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAACGCATCAAGGAAATCGACGATTT
CACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCGCACGGACGAATCCAGATGGAGAT
GGCTGTCTAGCGGAAATACAGAAGCTGGCTTTGATCCCGTTTTTCTTACTTATATATTTA
TACCAATTGATTGTATTTATAACTGTAAAAATGTGTATGTTGTGTGCATATTTTTTTTTG
TGCATGCACATGCATGTAAATAGCTAAAATTATGAACATTTTATTTTTTGTTCAGAAAAA
AAAAACTTTACACACATAAAATGGCTAGTATGAATAGCCATATTTTATATAAATTAAATC
CTATGAATTTATGACCATATTAAAAATTTAGATATTTATGGAACATAATATGTTTGAAAC
AATAAGACAAAATTATTATTATTATTATTATTTTTACTGTTATAATTATGTTGTCTCTTC
AATGATTCATAAATAGTTGGACTTGATTTTTAAAATGTTTATAATATGATTAGCATAGTT
AAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATAAACACAAATGATGTTTTTTCCTT
CAATTTCGATATCGAATTCCTGCAGCCCGGGGGATCCATTGAAAGGTTGCATCGTGAAAA
ATAGTTTAATAAAAAACGAAAAACAATAAATGAAGATCTATAAATATATAAATATTTGGA
TAAATAAAAATTATCTATATAAATAAGTAATATTATTTTCCCTCGGGATATATGCAATTT
AAAAAAATATTCTGATATTATTATATTTAAAATAATAAACAAAATTTATAAATATAAATA
GAAAATATTTTAAATATATTTCACAACAACAATAATGGTATAAGATAATAAAATAAAAAA
AAGAAAGAAAAATATATATTTCGATAAAATTTATTTCACATTGATTTAGATTATAAGTTA
ATTAATATAATAAAAAAATAAACATGTGTGTATCGTGTGTAATTCGGTATAAATATATTA
TGAAAATGTATTGCTAAATTGCAACAAATGAGCAATGTAGCTATACTAATTAAATAAAAA
AAAATCATAATATAGCTATATATTTTTATTTGTTAATACAAATCATATAAATTATGTACA
CGGCGTACTAATTTGTAGAAATACACATCTTACAAATATATATTTTTTTTAATAGCTGTC
TATCAAGCATAAATAATGAGTAATTAACATTAATTGTTCAGAAAAATAATATTCCTAAAA
ACGCTAGTTTAGTAGATAGGTGTGTATATACACATAAAAAGCTATGCATGTGTAAAGATG
GGATATGTATATCGCATAAAAAACATAAGTTTTCTTGCAAATTATACATATTTACCGTCA
GACATATTATTTATATTTTAAGAATATATTTTTTTTAGAATAGACACATTTTTAATTGTG
ATCAAATAAGCATTACGTTCACAATATTCATCAAATCCATAGTTTTAGGCGTGGGGAAAT
ATAAATAACAAATAATAATTTATAATTGTTCACGAAATTGATGAAATAAGAAACGATAAT
GCAAGTTTTAGTAAATCGAGTACGATAAGGCGGCCGCCACCGCGGTGGAGCTCCAGCTTT
TGTTCCCTTTAGTGAGGGTTAATTTCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCT
GTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCC
GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGG
AGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG
GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA
GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC
CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCAC
AAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC
CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTAT
CTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAG
CCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT
GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGT
ATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGC
AAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA
AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAAC
GAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC
CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT
GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCA
TCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCT
GGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCA
ATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC
ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG
CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCT
TCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA
AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTA
TCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGC
TTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCG
AGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAA
GTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG
AGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTC
ACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG
GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT
CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATA
GGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGT
TAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCG
GCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT
GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCT
ATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGT
GCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAA
AGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGC
TGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC
TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGC
GGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTT
GGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAAT
ACGACTCACTATAGGGCGAATTGGGTACCAATATTCAAATAATCACACAGGGTGAATTAT
CACTAAATACAAACAGCATGATTAAGTACCTTAATTGCATGCCTTTCTGTGAAAGCAAGC
AATTATATTCATTGTAAATATGGTACATTTGTTTGTGTATGCATTGGAAGGAAAATGGAA
TTAAGCGCATTCATAGGTAAATAAAAGATATATATATAAGAAAGTACACCTTTGCTACAA
ATAAAAGACAAGTTTTTGTTTTTAGCATAGGCACCTGGATGATCCACAAAAATGGCATTA
TTTTATAAGCATAAATATATGCTAAATATATATTCCAAGAAAACGTAATTTTTTGAGAAA
ATAAAATTGTTTAGAAAAATTGAAGAAATACAATAAACATATATTAAATTTTTATATACA
TTGAGGAATATGCATATTCTTTTGTTTTTGTGTATAAGAATAAATGATACATTTGATTTA
ATAAAATAAAAACAAATTATTAAATTGTTATTTTTGTGCTATTTTTATTAGTTTCATGCT
ATTTTCGTTTAATGTGTCAATATTTATTTTTTTATATAAGATGATATATCACCAAACTGT
TTTAAGCATTAGACTCTTACGTATAAAATGCTAATTTGTTGTTTTATAAAGTAATATTAA
TTAAATATCTTGTTTACACAAGGTATTAATTTGTTTAATATAAATAATATATACACATGT
ATATTTACATTGTCATTGCTATATTTTTAAATAATTGTACTATACCCAAAAATGTCATAT
TTCCATCATGCACAAAAAGGAAAAAAAACAAAAAAATAGCGGAAAAAAGTACTAGAGAAT
CCACTAAATAAATAAAAATGAAATATGTCTACAAATATATATACAACTAATACAAAATCA
CATATTTTTTCATAAAATATGGAAAAAAATAAGTGAAAATTCCGTATTATTATAAAACAA
AATTTTCTATTATATATTTATAAAATCGGTATTAGTTAGCATGATTATTATTAGGGATAT
TCCATTGCATCCATATCTAGAGCTTGCATGCCTGCAG
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1gatatcGCGGCCGCCTTATCGTACTCGATTTAC
Additional information primer 1L2420 (EcoRV/NotI); 5'
Sequence Primer 2GGATCCATTGAAAGGTTGCATCGTG
Additional information primer 2L2421 (BamHI); 5'
Sequence Primer 3ATCTAGATATGGATGCAATGGAATATCC
Additional information primer 3L2367 (XbaI); 3'
Sequence Primer 4TGGTACCAATATTCAAATAATCACACAGGG
Additional information primer 4L2368 (Asp718); 3'
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6