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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_1008500
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| Gene Model P. falciparum ortholog |
PF3D7_1436300
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| Gene product | translocon component PTEX150 |
| Gene product: Alternative name | PTEX150, Plasmodium translocon of exported proteins |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
|
| Type of plasmid/construct used | Plasmid double cross-over |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
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| Plasmid/construct map |
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| Plasmid/construct sequence |
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| Restriction sites to linearize plasmid |
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| Partial or complete disruption of the gene | Complete |
| Additional remarks partial/complete disruption |
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| Selectable marker used to select the mutant parasite | tgdhfr |
| Promoter of the selectable marker | pbdhfr |
| Selection (positive) procedure | pyrimethamine |
| Selection (negative) procedure | No |
| Additional remarks genetic modification | |
| Additional remarks selection procedure | In the paper it is reported that repeated attempts to generate gene knockouts in P. falciparum and P. berghei were unsuccessful. In the paper no details are provided on the constructs used to disrupt PTEX150. Information on the constructs to disrupt P. berghei PTEX150 provided here in the RMgm database are obtained from Dr. T.F. de Koning-Ward (The Walter &Eliza Hall Institute of Medical Research, Melbourne, Australia).
PTEX150 (Plasmodium translocon of exported proteins protein 150) is a protein with a putative ER signal, is expressed in blood stages; it has an apical location in merozoites and a parasitophorous vacuole membrane (PVM) localisation in ring forms and trophozoites. Evidence is presented that PTEX150 forms a complex with HSP101 (PF11_0175) and several other proteins in the PVM and functions as an protein trafficking apparatus (translocon) located in the vacuole membrane and involved in transport of proteins from the parasitophorous vacuole into the cytosol of the erythrocyte. |
Primer information: Primers used for amplification of the target sequences  Primer information: Primers used for amplification of the target sequences 
| Sequence Primer 1 | CGGGGTACC CTCTGTTGATGTTATAGATTGTCCAG |
| Additional information primer 1 | F1F |
| Sequence Primer 2 | CCGAAGCTT CACCATTCGATAATTTGTCAAAAGAC |
| Additional information primer 2 | F1R |
| Sequence Primer 3 | TCCGATATC CCGATGAGCTTGGAAATGGAAATACAG |
| Additional information primer 3 | F2F |
| Sequence Primer 4 | CGCGGATCC AAATGCGCCTATAAAGCATACGTATA |
| Additional information primer 4 | F2R |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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*RMgm-297