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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_0416700
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| Gene Model P. falciparum ortholog |
PF3D7_0904700
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| Gene product | bacterial histone-like protein |
| Gene product: Alternative name | PbHU |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
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| Type of plasmid/construct used | Plasmid double cross-over |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
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| Plasmid/construct map |
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| Plasmid/construct sequence |
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| Restriction sites to linearize plasmid |
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| Partial or complete disruption of the gene | Complete |
| Additional remarks partial/complete disruption |
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| Selectable marker used to select the mutant parasite | tgdhfr |
| Promoter of the selectable marker | pbdhfr |
| Selection (positive) procedure | pyrimethamine |
| Selection (negative) procedure | No |
| Additional remarks genetic modification | In the plastid, genomic DNA is assembled into a compact, highly organised structure, the nucleoid, like in bacteria from which the plastid has evolved. Bacterial nucleoid formation depends on a group of bacterial histone-like DNA binding proteins (BHLs). The HU protein, which binds DNA in a sequence non-specific manner and bends the bound DNA, is the most abundant BHL in the bacterial cell. The nuclear genome of P. falciparum encodes a HU homolog (PfHU) that is probably involved in DNA compaction in the plastid. PfHU is targeted exclusively to the parasite’s plastid and bound its natural target – the plastid DNA – sequence-independently. PfHU complements the lack of HU in Escherichia coli. |
| Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences  Primer information: Primers used for amplification of the target sequences 
| Sequence Primer 1 | 5’-ggtaccGCACATCTTTTGATTTTTTAGCCT-3’ |
| Additional information primer 1 | PbHU-F1-KpnI |
| Sequence Primer 2 | 5’-aagcttTTTGTTCTCTTGTATTTTAAGATA-3’ |
| Additional information primer 2 | PbHU-R1-HindIII |
| Sequence Primer 3 | 5’-gatatcTGCCTTTTATTGTAAAGAAAATAA-3’ |
| Additional information primer 3 | PbHU-F2-EcoRV |
| Sequence Primer 4 | 5’-ggatccTACCTTTAATACCCATTTGCATA-3’ |
| Additional information primer 4 | PbHU-R2-BamHI |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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*RMgm-266