Summary

RMgm-152
Malaria parasiteP. berghei
Genotype
Transgene
Transgene not Plasmodium: A fusion of GFP (gfp-mu3) and Luciferase Firefly (LucIAV)
Promoter: Gene model: PBANKA_0403200; Gene model (P.falciparum): PF3D7_0304600; Gene product: circumsporozoite (CS) protein (CSP)
3'UTR: Gene model: PBANKA_0719300; Gene product: bifunctional dihydrofolate reductase-thymidylate synthase, putative (dhfr/ts)
Replacement locus: Gene model: PBANKA_0306000; Gene product: 6-cysteine protein (230p)
Phenotype Sporozoite;
Last modified: 7 May 2018, 16:09
  *RMgm-152
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Introduction of a transgene
Reference (PubMed-PMID number) Reference 1 (PMID number) : 28348156
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
The mutant parasite was generated by
Name PI/ResearcherB. Franke-Fayard; C.J. Janse
Name Group/DepartmentLeiden Malaria Research Group
Name InstituteLeiden University Medical Center
CityLeiden
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-152
Principal name784cl1
Alternative namecspGFP-Luc
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot tested
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteStrong GFP-Luciferase expression in sporozoites.
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant expresses GFP-LucIAV under the control of the promoter of the circumsporozoite (CS) protein gene of P. berghei.

Phenotype
This mutant has not been characterized yet in detail, both with respect to (wildtype) growth characteristics and (strength) of the expression of GFP-Luciferase in oocysts, sporozoites and (young) liver stages.

Additional information
The transgene has been introduced in the non-essential 230p locus of P. berghei. Introduction of other (trans)genes into this locus has shown that disruption of 230p locus has no effect on the growth characteristics of the parasites.

Other mutants


  Transgene: Mutant parasite expressing a transgene
Type and details of transgene
Is the transgene Plasmodium derived Transgene: not Plasmodium
Transgene nameA fusion of GFP (gfp-mu3) and Luciferase Firefly (LucIAV)
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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1 tcgagaagag aaggaagact gacgacgcag ccactgcgga accgagcaac gcaatgagct ccttgacgtc cacgagggag
81 acaactcccg tgcacgggtt gcaggctcct tcttcggccg cagccattgc cccggtgttg gcgtggatgg acgaagaaga
161 ccggaaaaaa cgcgagcaaa aggaactgat tcgggccgtt ccgcatgttc actttagagg ccatgaagag ttccagtacc
241 ttgatctcat tgccgacatt attaacaatg gaaggacaat ggatgaccga acgggcgttg gtgtcatctc caaattcggc
321 tgcactatgc gctactcgct ggatcaggcc tttccacttc tcaccacaaa gcgtgtgttc tggaaagggg tcctcgaaga
401 gttgctgtgg ttcattcgcg gcgacacgaa cgcaaaccat ctttctgaga agggcgtgaa gatctgggac aagaatgtga
481 cacgcgagtt cctcgattcg cgcaatctcc cccaccgaga ggtcggagac atcggcccgg gctacggctt ccagtggaga
561 cacttcggcg cggcatacaa agacatgcac acagactaca cagggcaggg cgtcgaccag ctgaagaatg tgatccagat
641 gctgagaacg aatccaacag atcgtcgcat gctcatgact gcctggaatc ctgcagcgct ggacgaaatg gcgctgccgc
721 cttgtcactt gttgtgccag ttctacgtga acgaccagaa ggagctgtcg tgcatcatgt atcagcggtc gtgcgatgtc
801 ggcctcggcg tccccttcaa catcgcttcc tattcgcttt tgacgctcat ggttgcacac gtctgcaacc taaaacctaa
881 ggagttcatt cacttcatgg ggaacacgca tgtctacacg aaccatgtcg aggctttaaa agagcagctg cggagagaac
961 cgagaccgtt ccccattgtg aacatcctca acaaggaacg catcaaggaa atcgacgatt tcaccgccga ggattttgag
1041 gtcgtgggct acgtcccgca cggacgaatc cagatggaga tggctgtcta gcggaaatac agaagctagc tttgatcccg
1121 tttttcttac ttatatattt ataccaattg attgtattta taactgtaaa aatgtgtatg ttgtgtgcat attttttttt
1201 gtgcatgcac atgcatgtaa atagctaaaa ttatgaacat tttatttttt gttcagaaaa aaaaaacttt acacacataa
1281 aatggctagt atgaatagcc atattttata taaattaaat cctatgaatt tatgaccata ttaaaaattt agatatttat
1361 ggaacataat atgtttgaaa caataagaca aaattattat tattattatt atttttactg ttataattat gttgtctctt
1441 caatgattca taaatagttg gacttgattt ttaaaatgtt tataatatga ttagcatagt taaataaaaa aagttgaaaa
1521 attaaaaaaa aacatataaa cacaaatgat gttttttcct tcaatttcga tatcacataa aagggaatat ggaatatact
1601 agcatatact ccctgatata tttgttttaa taaaatgcat ttacaagcat cctcaatttt acaattcttt tttatttctc
1681 tatttatata tatgtgaatg tccgttttaa tataaacaat atattataaa ttgtatatta tatccgttgg tacaaatcgt
1761 gcattttgtg tcctcatgtt gcataaaaat agctaatttc ataaaccaat ttattatgat tgttcataaa aaaaaatata
1841 tttatctaga ttaataactt gggtttttgc aaactttacc aaatgaaaaa taactataat ttttatttaa ataattatat
1921 cataaattat tctttacatg tatagaaatg catatacaca aattagtaaa tatgagcacg cttttacttt gtccaggtat
2001 tatgctctta tttttagcat atatatattt atttatttat ttatatttgt gtgtatattc ccctacgtga caaatatgca
2081 agatggttat attttgtgca atgctaaaat ggggctatat gcacataaga gttgttctat catgtagtgt gctgccctta
2161 aaagataaaa ataattatat tttttttaat tcgagttttt ttaaaattat aataattttt caccatagac aaatgaaaaa
2241 atgctgaaaa catgcatata tatgtataaa ctttttttaa aatataaaaa ataattataa taagtgatat tatatatatc
2321 tgtggctata tatattagcc tgtatatatg tgtgcatata tatttctttt tcaccctcaa gttgggtaaa aataattaac
2401 aagtataaaa ttgttgagta atgtttatgc atgcatatat ttattatttg tttataatta aaataaaaaa ggtaccaaat
2481 attatatgca tatttttata ttgacatgca tatgtgttgg ttgtaattga ggaatataag gttgcgttat ggaaaatata
2561 aataatataa taatttgcat gcagtttttt atcaataaag agaagatcag ggcttgttgt aagatataat ataattctgc
2641 atgcaccttt ttttatttct atatcatgag cttattggaa tatgttctgc attaacaata tatcaacata tttatatata
2721 tatataaagc tataaatcgt tatattttca ttccaaaata tgttttaaat taaataaaaa ataattgata tacaattaaa
2801 aaatatataa cacatattta tataatattt tatttatttt attttttcta caattgtata tatgtaagca tatttggttt
2881 tatatataat attttgtact ttttcattta tatttttttc ttttttcgtt ttaaaatata tatttataac ccaaaataac
2961 gtttaatata tatatgtgaa caaattatcg taatatatat cttagtttat tttttttgat aaccctcaca taagacaatc
3041 cttataaaag gagttaaaaa aaaacattaa caaaaaacaa aatctatata tacacgcata tatttggatc catgagtaaa
3121 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
3201 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
3281 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
3361 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
3441 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
3521 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
3601 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
3681 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
3761 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaagggat cctggctagc
3841 cagtcgacct gcaggcatgc aagcttgcgg ccgatccaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
3921 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
4001 cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
4081 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
4161 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt
4241 cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
4321 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
4401 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat tcctctggat
4481 ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt
4561 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
4641 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca
4721 aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
4801 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
4881 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
4961 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga
5041 ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
5121 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt
5201 taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg
5281 ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
5361 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
5441 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
5521 atcgccgtgt aattctagaa gatcccgttt ttcttactta tatatttata ccaattgatt gtatttataa ctgtaaaaat
5601 gtgtatgttg tgtgcatatt tttttttgtg catgcacatg catgtaaata gctaaaatta tgaacatttt attttttgtt
5681 cagaaaaaaa aaactttaca cacataaaat ggctagtatg aatagccata ttttatataa attaaatcct atgaatttat
5761 gaccatatta aaaatttaga tatttatgga acataatatg tttgaaacaa taagacaaaa ttattattat tattattatt
5841 tttactgtta taattatgtt gtctcttcaa tgattcataa atagttggac ttgattttta aaatgtttat aatatgatta
5921 gcatagttaa ataaaaaaag ttgaaaaatt aaaaaaaaac atataaacac aaatgatgtt ttttccttca atttcgggta
6001 ccgagctcga attctcttga gcccgttaat gaaatagata caattcattc atgttatata catctagaac ataatctgaa
6081 tatggttcaa gttaaatgtc caaaaattat aaaaagtgat gatatttttg atggtaatac cataatagac accaaggtaa
6161 catcacgaag tagtcaacaa aataattttt atttagaaaa tacagatgtt gaaccagaag aaatagagaa atataaaaat
6241 atagaataca taccagaaaa cgatgaagta atgcatctag acaaaaaaga aaagctagat gatatattac caggtgttat
6321 catattagat aaaaataaaa tgttcaaaga aaaaggacat ttcacttttg ttactccatt aattgtagaa aaggtattaa
6401 tattaaaaat atattgtgat aatactaaaa caataattaa taatatgaaa gggaaaaaag gtattacagt aataaggatt
6481 tctcaaaata caacaaaaaa taaattttat ggatgtgact tttcaggtaa ttctaaaaaa acattttact attccaatgt
6561 ttatgattta gaaaaaaaaa atgagttttg tgaaatagaa ttaaaagaaa atatagtagt tagcttaaat tgtccaactg
6641 gtaaaattaa tccaaaaaat tgttttagaa atgtatatat aaaaagtaat atgaatgaac aaacaaccga aaatatagaa
6721 aatatattta acgaaataaa agttatagat gcagattatt ttataaataa ttcatcaacc tttttgatga tttccaaaat
6801 tacaaaaaaa gagtttgatt tttattgtac atgtgaagat tataaaacca aaaatatagg aacaatatat attaaaaatt
6881 atgaatatct agattcaaaa cctaaatata aaaataaaca aatttcctat atagatgtag ttccataccc gcggggaaag
6961 ggcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
7041 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
7121 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
7201 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
7281 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
7361 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
7441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
7521 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
7601 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
7681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
7761 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
7841 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
7921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
8001 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
8081 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
8161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
8241 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
8321 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
8401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
8481 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
8561 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
8641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
8721 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
8801 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
8881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
8961 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
9041 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
9121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
9201 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
9281 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
9361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
9441 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
9521 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
9601 gcttccgcgg gtatatggta aagaacctac taacacaata aaatatttaa ataatgtatt tcctataaat aaatttacag
9681 atttattttt taatacaaaa gatatagata taccagaaat aaatgatcag tttaaaggtt ttaaattctt tatgacatca
9761 tttataaatc atggatcata tccactaaca atagaatgtg gtgtaacaaa tggtggaact agttataaaa gagcaattat
9841 tttattgcat gttcgaactg atttaaaaga tagaccagtt tcattttgtg attttcgaaa aggagaatta tataattatt
9921 tgaatgctta tactgaaggg gatgtatgca taataatttc caaatcaaat acaagttttg gttttagatg cccagtaaat
10001 acaaaaaaaa tgccaaaaaa ttgttttacg caagtatatg aaaaagggta tctaaatgac gccaataaaa ttaatactaa
10081 aaatgttatt aactattcat ttgaaaatcc agaatatgcg ctagctggtt ytaattatac attaacaaaa tcgtatcaat
10161 ttgaatgtca ttgtgtagat aaagaaacag aacaaattgt aaaaacggtt ttagtcaaat atgtaaatga agatgaaata
10241 tatgattata atgattttcc aatggtgaat cacaaaccta ttattgcaca tccaaataaa acacatcaag cttgcatgcc
10321 tgcaggtcaa caataaataa taaataaata ttgtggaaat aaaataacat ataattattt ttaatacatt gatttccctt
10401 ttattttttt aaatttcatt gatataaaaa tatataataa taacatatat gatttcaaat taatcttttc aaaaatggtg
10481 tttatttttg tatgtttgtg tatgaattaa tcacataaca catctattaa attgagttgg taatatagac acaaataaat
10561 atatatattt ttatagctta aaagtgtgtt atgaatattt taagcatatt ttctttttct ttggattgtg taaaatgaac
10641 tcatataatg cgtttttttg tttttgttat tttgtcattt tgttattttg ctattttatg gattaatttt tgtttataaa
10721 atgggaaata attttaacat atttaaataa atggagaaaa aatataaaat aattataaaa aaaagttaat acacattttt
10801 tcctgttata gaccttatat ttatttatcc atatatatat atatatatat atatatatat atatacatac caagtgaatt
10881 aagaggaaag ctaatttatt attcagaata atatatgaac tatatataat ttttattatt ttggtgtata ttaatctgtc
10961 tatatgcata catgcaataa tttatcgact tatatatcaa ataacataaa atagaagtgt tttaaattat ggatatatgc
11041 tcaatattca ttttttttaa taagttagct atatttaaat tatacatttt atatatggtc tctttttttt ttaaatatta
11121 tttaagtgat catgaaaata taaataattt ttttttattt aatatccttt tgcttgcatg tggtaaatgg aaatttggat
11201 gtgttttgaa rgttcggata tagttgtatg gacatataat atattttgtg aaaaattggt tttatgttta tacttatgcc
11281 aatacttttt gagtaaaaca aagcaagtgc ttataaataa ttaaagccaa ttttataata tatatttttt tatttaattt
11361 gaatttagta gtataatttt ttatggtaag tgctcaaaga gagttgctta taaagtatgg tttgtttctt tttcgccatt
11441 ttgaattaca cattaaaaat atatagatac atatattata atatgaaatc attaataatt tagggaaatt ctacaaattt
11521 aaaaacgaat aaaataattg tttttcatca tgccataaca caatattgat atatacatgt acaaacattt tttttatttg
11601 gaaaatataa attatataaa aaaaaatgta tagtatacaa aatgagcata ttcacacggg gtggacgttc attttttcat
11681 ttttcccctg ttttttatga gtatatgata aaattttatg aacatttaca caaaatgaaa atggatatat aggaaaaatg
11761 gagcggtatt tcatttatct ttgattgtca tttggatatt atattaccyt gggtaggcaa ttaaaaatgt taaataacaa
11841 tttaaggaaa ttatatttta tatattaaaa ttaacactgt attatatgat tcgcttataa aagccactct ttccccatgc
11921 aaagctgttt aatatcaatt ttaacaaatt acacacatgt taatatattt atatatataa tttatatatt tataatttat
12001 atatttatat ttttattatt tatatattta ttatttattg tgtgtgtcaa ttcgggtagg atatacctct tttttattgt
12081 ttaaagcgat ttgtattcta aaatataaag ratttgaaaa agagaaagat agaatatgat cccatcatat atagccctat
12161 aatttttatt tagcagcgaa ttaatttttc tattaagttt atgtgtaatt aaaataacgg aatatatata atacaataaa
12241 aaagtgcata aattaaaatt ttttcaatta aatttttttt tttaaggggt tatataatat taaatatata aaatacgatt
12321 atatattttt gctacaattt tttatattaa gatataaata gtaaataaat ggtattatat ggcatgtaat atataaattt
12401 tttccaattt ttattttata tacacttttc ctttttttgt cataaaactt aaacaattta cacattcatt ttaaaaattg
12481 actatttgtt tcaacatttt ttgagtttcc gttttataat agtattttca tttgtatatt gcttatatat ataaatacac
12561 acctaaatgt tacaaaggat caatgcataa accggtgtgt ctggtcgtcg cgatgacccc caagaggggc atcggcatca
12641 acaacggcct cccgtggccc cacttgacca cagatttcaa acacttttct cgtgtgacaa aaacgacgcc cgaagaagcc
12721 agtcgcctga acgggtggct tcccaggaaa tttgcaaaga cgggcgactc tggacttccc tctccatcag tcggcaagag
12801 attcaacgcc gttgtcatgg gacggaaaac ctgggaaagc atgcctcgaa agtttagacc cctcgtggac agattgaaca
12881 tcgtcgtttc ctcttccctc aaagaagaag acattgcggc ggagaagcct caagctgaag gccagcagcg cgtccgagtc
12961 tgtgcttcac tcccagcagc tctcagcctt ctggaggaag agtacaagga ttctgtcgac cagatttttg tcgtgggagg
13041 agcgggactg tacgaggcag cgctgtctct gggcgttgcc tctcacctgt acatcacgcg tgtagcccgc gagtttccgt
13121 gcgacgtttt cttccctgcg ttccccggag atgacattct ttcaaacaaa tcaactgctg cgcaggctgc agctcctgcc
13201 gagtctgtgt tcgttccctt ttgtccggag ctcggaagag agaaggacaa tgaagcgacg tatcgaccca tcttcatttc
13281 caagaccttc tcagacaacg gggttcccta cgactttgtg gttc
Restriction sites to linearize plasmid KspI (SacII)
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationThe GFP-Luciferase gene (1 copy) has been inserted into the 230p locus (PB000214.00.0) by double cross-over integration.
Additional remarks selection procedure
Other details transgene
Promoter
Gene Model of Parasite PBANKA_0403200
Gene Model P. falciparum ortholog PF3D7_0304600
Gene productcircumsporozoite (CS) protein
Gene product: Alternative nameCSP
Primer information details of the primers used for amplification of the promoter sequence  Click to view information
Primer information details of the primers used for amplification of the promoter sequence  Click to hide information
Sequence Primer 1CCGGATATCACATAAAAGGGAATATGGAATATACTAGC
Additional information primer 1EcoRV
Designed on primers described in Frevert, U. et al, 2005, PloS Biol 3, e192
Sequence Primer 2CGCGGATCCAAATATATGCGTGTATATATAGATTTTG
Additional information primer 2BamHI
Designed on primers described in Frevert, U. et al, 2005, PloS Biol 3, e192
3'-UTR
Gene Model of Parasite PBANKA_0719300
Gene productbifunctional dihydrofolate reductase-thymidylate synthase, putative
Gene product: Alternative namedhfr/ts
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to view information
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Insertion/Replacement locus
Replacement / InsertionReplacement locus
Gene Model of Parasite PBANKA_0306000
Gene product6-cysteine protein
Gene product: Alternative name230p
Primer information details of the primers used for amplification of the target sequences  Click to view information
Primer information details of the primers used for amplification of the target sequences  Click to hide information
Sequence Primer 15'-cccaagcttccgcgggtatatggtaaagaacctactaacac
Additional information primer 1primer L1345: 0.7kb 5'region of PB000214.00.0 (230p gene)
Sequence Primer 25'-cccaagcttgatgtgttttatttggatgtgc
Additional information primer 2primer L1346: 0.7kb 5'region of PB000214.00.0 (230p gene)
Sequence Primer 35'-ccggaattctcttgagcccgttaatg
Additional information primer 3primer L1347: 1kb 3'region of PB000214.00.0 (230p gene)
Sequence Primer 45'-tccccgcgggtatggaactacatctatatagg
Additional information primer 4primer L1348: 1kb 3'region of PB000214.00.0 (230p gene)