SummaryRMgm-1500
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene disruption |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 27427910 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Kehrer J; Mair GR |
Name Group/Department | Integrative Parasitology, Center for Infectious Diseases |
Name Institute | University of Heidelberg Medical School |
City | Heidelberg |
Country | Germany |
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Name of the mutant parasite | |
RMgm number | RMgm-1500 |
Principal name | Δpat |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Male and female gametes fail to egress from their host erythrocytes after induction of gametogenesis. |
Fertilization and ookinete | Not tested |
Oocyst | No oocyst formation |
Sporozoite | No oocyst and sporozoite formation |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation A mutant lacking expression of PAT (Δpat; RMgm-1500); A mutant in which the endogeneous pat gene is replaced with the pat gene of P. falciparum 3D7: Δpat;pat(PF3D7)::mcherry; RMgm-1501) A mutant expressing GFP-tagged PAT and mCherry-tagged G377 (pat::gfp;g377:mcherry; RMgm-1503): A mutant lacking expression of PAT and expressing mCherry-tagged G377 (PBANKA_1463000) and a mutant lacking expression of PAT and expressing mCherry-tagged PPLP2 (PBANKA_1463000) A mutant expressing a GFP-tagged version of TRAP (ss::gfp::trap; RMgm-1505) A 'promoter-swap' mutant expressing PAT under the promoter of the ccp1/lap2 promoter (PBANKA_1300700)(patccp.PP; RMgm-1506) |
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Details of the target gene | |||||||||||||||||||||||||
Gene Model of Rodent Parasite | PBANKA_0303900 | ||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_0206200 | ||||||||||||||||||||||||
Gene product | pantothenate transporter | ||||||||||||||||||||||||
Gene product: Alternative name | PAT | ||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||
Type of plasmid/construct used | (Linear) plasmid double cross-over | ||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||
Partial or complete disruption of the gene | Complete | ||||||||||||||||||||||||
Additional remarks partial/complete disruption | |||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | tgdhfr | ||||||||||||||||||||||||
Promoter of the selectable marker | pbdhfr | ||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||
Additional remarks genetic modification | |||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
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