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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1349800
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Gene Model P. falciparum ortholog |
PF3D7_1335900
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Gene product | thrombospondin-related anonymous protein | sporozoite surface protein 2 |
Gene product: Alternative name | sporozoite surface protein 2; SSP2; SSP-2; TRAP |
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Details of the genetic modification |
Short description of the mutation | The cytoplasmic tail domain (CTD) of TRAP replaced with the CTD of TLP (Trap-Like Protein (AY484471) |
Inducable system used | No |
Short description of the conditional mutagenesis | Not available |
Additional remarks inducable system |
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Type of plasmid/construct | Plasmid single cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | pbdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | The construct used results in replacement of the wild type trap-gene with a mutated trap gene. In the mutated trap gene the cytoplasmic tail domain (CTD) of TRAP is replaced with the CTD of TLP (Trap-Like Protein (AY484471). The CTD of TLP was obtained using the following primers: PbTLPfor (5' CGGGATCCAAAAACAAACAAATAATTCCAACTAGC3'; and PbTLPrev (5' TGCTCTAGATCATTTCCATGGAGAATTGTCATTATAATC 3'). The mutated TRAP gene is under the control of the 5'-UTR of TRAP and the 3'-UTR of pbdhfr. |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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