Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of PBANKA_1346300

Protein (function)
Members of the CPW-WPC family of proteins are characterized by the presence of one or more arrays of the CPW-WPC domain. Approximately 60 amino acids long, the domain is named for two conserved motifs; namely, CPxxW proximal to the amino-terminus and WPC proximal to the carboxy-terminus of the domain. In some versions of the domain tryptophan is replaced with another aromatic residue, tyrosine or phenylalanine. Each domain typically contains 6 cysteines, likely paired in disulfide bridges. The cpw-wpc gene family is widely conserved among apicomplexans, including Plasmodium, Theileria, Babesia, Toxoplasma, Eimeria and the gregarines. The CPW-WPC proteins are absent in Cryptosporidium, suggesting that these genes were lost following the split of the cryptosporidian lineage. Plasmodium CPW-WPC proteins possess a variable number of CPWWPC domains, ranging from 1 to 7; and they are randomly distributed across the protein with no preference for either the amino or carboxy terminus. The cpw-wpc genes are conserved as orthologs across Plasmodium. The cpw-wpc gene family has 9 members in P. falciparum, distributed across various chromosomes with some members encoded within a single exon (PF3D7_0320200 and PF3D7_1331400) while others are disrupted by 9 or more introns, with as many as 15 introns disrupting the ORF of PF3D7_1429300. In P. berghei the gene structures are conserved with equal numbers of exons encoding each ortholog. The individual Plasmodium members differ in length from 178 to 1256 amino acids.

Phenotype
No expression of the protein in gametocytes. Expression in ookinetes with evidence for a location in the crystalloid organelle.

Additional information
Evidence is presented for translational repression of transcripts of this gene in gametocytes.

Other mutants
See the link CPW-WPC for transgenic/mutant lines targetting one of the other members of the CPW-WPC family.
See RMgm-1458 for a mutant expressing a C-terminal tagged version of another family member (PBANKA_1352500)