| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 27241521
|
| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Kumar H; Frischknecht F |
| Name Group/Department | Integrative Parasitology |
| Name Institute | Center for Infectious Diseases, Heidelberg University Medical School |
| City | Heidelberg |
| Country | Germany |
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| Name of the mutant parasite |
| RMgm number | RMgm-1449 |
| Principal name | UIS3(–) |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Not different from wild type |
| Sporozoite | Not different from wild type |
| Liver stage | Normal numbers of sporozoites are formed that are infective to cultured hepatocytes. Parasites arrest during development in hepatocytes after a period of growth. In most parasites no merozoites are formed as shown by the low level of MSP1 expression. After intravenous injection of sporozoites, 6 out of 46 mice developed a blood stage infection. |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of UIS3
Protein (function)
UIS3 has been identified by transcription-profiling of sporozoites (Kaiser et al., (2001). Mol. Microbiol. 51, 1221-32).
Phenotype
Normal numbers of sporozoites are formed that are infective to cultured hepatocytes. Parasites arrest during development in hepatocytes after a period of growth. In most parasites no merozoites are formed as shown by the low level of MSP1 expression. After intravenous injection of sporozoites, 6 out of 46 mice developed a blood stage infection (see also below).
Additional information
The mutant described here show a less strong growth-arrested phenotype in comparison to an earlier described mutant lacking expression of UIS3 (see RMgm-36), possibly due to strain differences (as suggested by the authors). The latter mutant was made in parasites of the NK65 strain of P. berghei. The mutant described here is made in parasites of the the ANKA strain.
Other mutants
See the link PBANKA_1400800 for other related mutants |
*RMgm-1449
