Summary

RMgm-1449
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_1400800; Gene model (P.falciparum): PF3D7_1302200; Gene product: early transcribed membrane protein 13 (UIS3)
Phenotype Liver stage;
Last modified: 2 June 2016, 18:18
  *RMgm-1449
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 27241521
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherKumar H; Frischknecht F
Name Group/DepartmentIntegrative Parasitology
Name InstituteCenter for Infectious Diseases, Heidelberg University Medical School
CityHeidelberg
CountryGermany
Name of the mutant parasite
RMgm numberRMgm-1449
Principal nameUIS3(–)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNormal numbers of sporozoites are formed that are infective to cultured hepatocytes. Parasites arrest during development in hepatocytes after a period of growth. In most parasites no merozoites are formed as shown by the low level of MSP1 expression. After intravenous injection of sporozoites, 6 out of 46 mice developed a blood stage infection.
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of UIS3

Protein (function)
UIS3 has been identified by transcription-profiling of sporozoites (Kaiser et al., (2001). Mol. Microbiol. 51, 1221-32).

Phenotype
Normal numbers of sporozoites are formed that are infective to cultured hepatocytes. Parasites arrest during development in hepatocytes after a period of growth. In most parasites no merozoites are formed as shown by the low level of MSP1 expression. After intravenous injection of sporozoites, 6 out of 46 mice developed a blood stage infection (see also below).

Additional information
The mutant described here show a less strong growth-arrested phenotype in comparison to an earlier described mutant lacking expression of UIS3 (see RMgm-36), possibly due to strain differences (as suggested by the authors). The latter mutant was made in parasites of the NK65 strain of P. berghei. The mutant described here is made in parasites of the the ANKA strain.

Other mutants
See the link PBANKA_1400800 for other related mutants


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1400800
Gene Model P. falciparum ortholog PF3D7_1302200
Gene productearly transcribed membrane protein 13
Gene product: Alternative nameUIS3
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6