RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-1444
Malaria parasiteP. yoelii
Genotype
TaggedGene model (rodent): PY17X_0823700; Gene model (P.falciparum): PF3D7_0919500; Gene product: major facilitator superfamily domain-containing protein, putative (MFS3)
Name tag: triple-HA
Phenotype Sporozoite; Liver stage;
Last modified: 6 May 2016, 20:09
  *RMgm-1444
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 27128092
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. yoelii
Parent strain/lineP. y. yoelii 17XNL
Name parent line/clone Not applicable
Other information parent line
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The mutant parasite was generated by
Name PI/ResearcherSwearingen KE; Kappe SH; Sinnis P
Name Group/DepartmentJohns Hopkins Malaria Research Institute and Department of Molecular Microbiology and Immunology
Name InstituteJohns Hopkins Bloomberg School of Public Health, Johns Hopkins University
CityBaltimore
CountryUSA
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Name of the mutant parasite
RMgm numberRMgm-1444
Principal nameHA(3x)-PY17X_0823700
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
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Phenotype
Asexual blood stageNot tested
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteThe staining and localization of MFS3 in sporozoites co-localized with CSP, suggesting surface localization.
Liver stageIn cultured hepatocytes, the localization of MFS3 on the parasite plasma membrane located just interior to the PVM (most evident at 8 and 16 h post-infection)
Additional remarks phenotype

Mutant/mutation
The mutant expresses a C-terminal 3xHA-tagged version of MFS3

Protein (function)

Phenotype
The staining and localization of MFS3 in sporozoites co-localized with CSP, suggesting surface localization. In cultured hepatocytes, the localization of MFS3 on the parasite plasma membrane located just interior to the PVM (most evident at 8 and 16 h post-infection).

Additional information
MFS3 is a putative suger transporter?

Other mutants
RMgm-1384: a P. berghei mutant lacking expression of MFS3 (reduced sporozoite production)

  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PY17X_0823700
Gene Model P. falciparum ortholog PF3D7_0919500
Gene productmajor facilitator superfamily domain-containing protein, putative
Gene product: Alternative nameMFS3
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Details of the genetic modification
Name of the tagtriple-HA
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: StudioDrie StudioDrie; S. Mededovic and A. van Wigcheren