RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-1403
Malaria parasiteP. berghei
Genotype
Genetic modification not successful
TaggedGene model (rodent): PBANKA_1115300; Gene model (P.falciparum): PF3D7_0515700; Gene product: glideosome-associated protein 40, putative (GAP40)
Name tag: mCherry-3xMyc
PhenotypeNo phenotype has been described
Last modified: 5 April 2016, 12:11
  *RMgm-1403
Successful modificationThe gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted Gene tagging
Number of attempts to introduce the genetic modification 2
Reference (PubMed-PMID number) Reference 1 (PMID number) : 26796412
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255)
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Attempts to generate the mutant parasite were performed by
Name PI/ResearcherKooij TWA; Kenthirapalan S
Name Group/DepartmentMolecular Parasitology - Departement of Medical Microbiology
Name InstituteRadboud University Medical Centre
CityNijmegen
CountryThe Netherlands
  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1115300
Gene Model P. falciparum ortholog PF3D7_0515700
Gene productglideosome-associated protein 40, putative
Gene product: Alternative nameGAP40
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Details of the genetic modification
Name of the tagmCherry-3xMyc
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: StudioDrie StudioDrie; S. Mededovic and A. van Wigcheren