Mutant/mutation
The mutant lacks expression of NPT1 and expresses GFP under control of the constitutive hsp70 promoter

Protein (function)
Membrane transport proteins (MTP) transfer compounds across biological membranes and encompass diverse gene families, namely ion channels, ATP-dependent pumps and secondary active porters including those of the major facilitator superfamily. In contrast to bacteria, archaea and fungi, parasitic protozoa such as Trypanosoma brucei and the malaria parasite Plasmodium falciparum allocate only a small proportion of their genomes (2–3%) to membrane transport. P. falciparum encodes at least 122 MTPs. For 39 gene products, functional or subcellular localization predictions remain elusive, rendering them orphan MTPs.
In this study a comprehensive genetic analysis is reported of 35 orphan transport proteins of Plasmodium berghei during its life cycle in mice and Anopheles mosquitoes.

Phenotype:
Strongly reduced exflagellation; No sporozoites in vivo; No transmission by bite of infected mosquitoes;

Additional information

Other mutants