RMgmDB - Rodent Malaria genetically modified Parasites

RMgm-1331

Malaria parasite	P. berghei
Genotype
Genetic modification not successful
Tagged	Gene model (rodent): PBANKA_1008500; Gene model (P.falciparum): PF3D7_1436300; Gene product: translocon component PTEX150 (PTEX150) Name tag: mCherry-3xMyc
Phenotype	No phenotype has been described

Last modified: 5 April 2016, 09:48

*RMgm-1331

Successful modification	The gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted	Gene tagging
Number of attempts to introduce the genetic modification	3
Reference (PubMed-PMID number)	Reference 1 (PMID number) : 26219962
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Parent parasite used to introduce the genetic modification
Rodent Malaria Parasite	P. berghei
Parent strain/line	P. berghei ANKA
Name parent line/clone	P. berghei ANKA cl15cy1
Other information parent line	A reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
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Attempts to generate the mutant parasite were performed by
Name PI/Researcher	Matz, JM; Kooij, TW
Name Group/Department	Medical Microbiology
Name Institute	Radboudumc
City	Nijmegen
Country	The Netherlands

Tagged: Mutant parasite with a tagged gene

Details of the target gene

Gene Model of Rodent Parasite

PBANKA_1008500

Gene Model P. falciparum ortholog

PF3D7_1436300

Gene product

translocon component PTEX150

Gene product: Alternative name

PTEX150

Details of the genetic modification

Name of the tag

mCherry-3xMyc

Details of tagging

C-terminal

Additional remarks: tagging

Commercial source of tag-antibodies

Type of plasmid/construct

(Linear) plasmid double cross-over

PlasmoGEM (Sanger) construct/vector used

Modified PlasmoGEM construct/vector used

Plasmid/construct map

Plasmid/construct sequence

Restriction sites to linearize plasmid

Selectable marker used to select the mutant parasite

hdhfr/yfcu

Promoter of the selectable marker

pbdhfr

Selection (positive) procedure

pyrimethamine

Selection (negative) procedure

Additional remarks genetic modification

Additional remarks selection procedure

Primer information: Primers used for amplification of the target sequences Click to view information

Primer information: Primers used for amplification of the target sequences Click to hide information

Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6