RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-1247
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_1400300; Gene model (P.falciparum): Not available; Gene product: PIR protein; BIR protein (PIR1)
Name tag: mCherry
TaggedGene model (rodent): PBANKA_0500200; Gene model (P.falciparum): Not available; Gene product: BIR protein; PIR protein (PIR3)
Name tag: EGFP
Transgene
 Transgene not Plasmodium: A fusion of GFP (gfp-mu3) and Luciferase Firefly (LucIAV)
Promoter: Gene model: PBANKA_0915000; Gene model (P.falciparum): PF3D7_1133400; Gene product: apical membrane antigen 1 (ama-1)
3'UTR: Gene model: PBANKA_0719300; Gene product: bifunctional dihydrofolate reductase-thymidylate synthase, putative (dhfr/ts)
Replacement locus: Gene model: PBANKA_0306000; Gene product: 6-cysteine protein (230p)
Phenotype Asexual bloodstage; Liver stage;
Last modified: 18 November 2016, 13:10
  *RMgm-1247
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging, Gene tagging, Introduction of a transgene
Reference (PubMed-PMID number) Reference 1 (PMID number) : 27851824
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone RMgm-1281
Other information parent lineA transgenic line expressing a mCherry-tagged version of PBANKA_1400300 (PIR protein; PIR1) and expressing the fusion protein GFP-luciferase under the control of the ama1 promoter
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The mutant parasite was generated by
Name PI/ResearcherA. Fougère, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentLeiden Malaria Research Group
Name InstituteLeiden University Medical Center
CityLeiden
CountryThe Netherlands
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Name of the mutant parasite
RMgm numberRMgm-1247
Principal name2020; 2021
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
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Phenotype
Asexual blood stageThe two tagged-protein are exported into the cytoplasm of the host erythrocyte and show a diffuse or patchy localization in the cytoplasm.
1-5% of blood stages expressed both proteins simultaneously in a single infected erythrocyte
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stagePIR1 is expressed in late liver stages (schizonts) where it is located in the parasitophorous vacuole.
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information

The mutant parasite expresses  mCherry-tagged PBANKA_1400300 (PIR1), GFP-tagged PBANKA_0500200 (PIR3) and expresses the fusion protein GFP-luciferase under the control of the ama1 promoter

The pir3::gfp DNA construct was introduced in mutant RMgm-1281 that contains a mCherry-tagged version of pir1 and gfp-luciferase in the 230p locus (under the control of the ama1 promoter)

The proteins have been selected for tagging in a screen for putative exported proteins of P. berghei. The genotype of the parasites has not been analysed in detail. Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct genes. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype of different life cycle stages show:
- The two tagged-proteins are exported into the host erythrocyte and show a diffuse or patchy localization in the cytoplasm. 1-5% of blood stages expressed both proteins simultaneously in a single infected erythrocyte.
- No expression in oocysts and sporozoites could be detected
- PIR1 is expressed in late liver stages (schizonts) where it is located in the parasitophorous vacuole.




Other mutants
See PBANKA_1400300 and PBANKA_0500200 for other mutants expressing these proteins

  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1400300
Gene Model P. falciparum ortholog Not available
Gene productPIR protein; BIR protein
Gene product: Alternative namePIR1
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Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesClontech
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC
ATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGA
GATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTC
CAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACC
CTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG
CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAA
AGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCAC
CACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCG
CAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGAGCTCCA
CCGCGGCGATAATTATAAATAATATGGTAGATTGAATAGCGTTATTGTTCTAATATATAT
AATATTGTATCAATGATTAAAACTGAAATATGTTAAATTTGGGAAACATGTAAATTATAT
ATTAATTCAAAGTATACAGAAAAGGTATGTAATAATTAATTTAATTAGAATAATATTTTT
ATTAGGTTATTATATATATATATAAAATCACAAATATATTGTTATTGCTAAATAATATAT
ATTCTAAAATACTTAATTTAAAAACTATGAAACGAGGGAATATTATACATTGGTTTAAAG
TATTTTTATTAAGCGCATTAATTATTCCGTTGTAATGTACAGTGATATAGTAGTAACACT
AATAATAGTAGTAATAGCAATAGATAAACGTATTAAATACGAACAAATCATTAAATTCAT
TTGATTCGAATACGTTGATATATATATTAATTATATATATTATTAAACTTATAATAATAT
TAAAATTGTATGTATAAAAGATCAAATGAAATATTATAATACTTATTTAAGTAAGCTTTA
ATGCGATAATATTATAATTAACACTGCCAAGCAAAATAATATTAATAATTCTATAGTTTT
CCATCATAGAACTAAATATAGTTTATTATAAAATATAAAAGCAATCTATATATTTAGAAA
ATAATTCTAAGGCATTTTTAATGTATGCATGAATTGAAAGCTTTAATGGTAGAAAACATA
AAACATAATTAAACTATTTAGAATAAGTAAATCTTATATGGCTATATCCTTGTCTTAAAA
AACATACTTCCCTTATCTCTCCTCTCAATGTGCAATATACCAATCTAATACACATAGTTT
TCTATTATACTTTAAAATTTGCATCAATAATTATTTATGTGTTATATATTTAATATATAC
TAAGTATTATTTTAAAAAAAATATGCATTTAAAGACATATTTATGCTTATAAATAACATA
ATAAATACGGTTTTAGTGCTAATTGCCGTTTTAAACCGTGGGAAATATGTGCAAAATATA
TTATAAAATCACCCAATAGTGTATATTTTTAAATTGAAGTATATATGAATAAATATAAAG
TTATTTATTTATTAAAATGGACTATGAATTTATTCATATTAAATAAAAATAATGTTAAAA
TTATGATATGCAATTAAAAAAGGGAGCAATGCAACTTATTTTGTAAATGTTTTAATTTTA
GAAAAAACTATATTATATATTATAAGAAACAAGTATATACATATTTAATATATTTAAAAA
AATTACTATTTATATACTTTTAAGGATTATAGTAATAATAGGATTTTTTATTTTTTAGAT
TTATACATTATTTAAGTTTCAGGACATTGTTTATGTTCTATATTAAAGCATATGTATAAG
ATATATTTTAAATTCATTATAAAAGTATATTAATTTTTATAATAATGGTCATATGAGTGT
TATTATGGATAAAAATTTATGCAAAATTGTATTATATATACATATGATAATAAATGTTTA
AACAAACCCTCAAAATATGATAATTTATCTAACTACCATTAAAGTATATATTGTTCCATA
TTATCTTTAAATTTATATTAAGAATAATAAAAATAATTTTATTTACAGAAATTTGCTGTA
TATAGGGTTAAATAATTGTATTACCTATTTTAGTATATATATAACATGATATCCCCTTAT
CCTAGAAACTATAAATTATATTCATCATAATGGATGATGCTCTAGTATGTACATTTTTTT
ATGTTATATTTTCTATAATCTTCATTAATTTTTATTTTAATATCATTTTCTTAATACATA
TTTTTATCATTTTTTTCTAAATGGTTTCTTAGTGTCAAAACTTTGATGTTTTGAGGATGC
ATTTACCCGCTAAATTAGGCGGAACCGAAAAATGTGAACTTAAAAATATTAGTGATTTCC
AAAATTACTGCCCTAATAAAAACTGCAATACTGATCTCGAAAAAATTACGGTTGGATTTT
TATGGTTACTTGGAAAATATTATTCTATATCCCAAAATAGAAGTTATAATGAAAATAATA
CTAATCCATTTTTTCTATATATGATTTCATGGTTTAGTTACAAATTAAGTCAAATCACAG
GGCACGATTCCACCACAATAAACGATTTTTATAATAAAAATATAATAAATAATGATAAAT
ATGAAAAATTTATAAGTGATTCCAGTAAATTTACAAAACTTAATGAAGAATTAAATACAA
AAAGTGATTTTTTGAATATTAATATTGAAGATCTGTCTAAATTTTATGATGCATTTAAAC
TATTATGTAGTATGCATGATAAAGTTGCAAGGAATCAAACAGGCGACATACTGTTAAATA
ATGCTACTAGTTTTGTTGAGAAATATACAGAGCTAAACAATAGATATAATAATGGTATCC
CACATACTAAAATATTGTCTGTTTTATTAACTGGTTATAATAATTTAAAAAATGATCATA
CATATTTTCCATCCATTCTAACAATAGAACAAAAACATCATGTACAAAGTTCTGGAGGTA
ATTATGGACAAAGTTCTGAACTAATTTCTGAAATTACATCAAGTTCGTCGATAGGAAACA
AATTATTTACAGTTTTATCGATATTTGGTGCAATAGCATTTTTTTTAGGAATTTCTTATA
AGGTAAATAATAAGGAATTTAAAAATAATTTTCATTATATATATAGAAATGTTAACAAAA
AAATCATACGTTTCTTAACATTTTATATTAGTATTCGTTATTTGGATTTCGGAAACGAGT
TCAAAAACAACATTTAAGAGGAACGTTAAAAAATATAAAGAAGAAAATGAATTATCGGGG
ATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT
CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGA
GATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGT
GACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGG
CTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCC
CGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGAC
CCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAA
CTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGA
GCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAA
GGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCA
GCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTA
CACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGA
GCTGTACAAGTAATCTAGAATTATTAATATATATGAATATATATACATCGTTGTATGCGT
AATATACGTGTAACTGTTTTTTTTAATTTTTATATTAACTTTTCCTTATACATTTTAATG
CCATTAACTAATAATATTTTACTTGAAAAAATTCTAAATTTTTATAACTCTAATATAAAA
TCTGTGAATAATAAAAAATTAGCATAAAGTACGAATTAAACATCGAATATTTGTTTTAAA
GGGGCACACATTATGTGCATATAATTTTATTCTTTGAATTTAATTTTTTGCTTATAATTA
GATTAAATTAATATAATTTTTTTATTGAAAATAAAAACTTATTTTAAAAAATTAACAAAT
GGAAGTTAATGAAAAATACGAAAAAAGTAAAGGGTTAATTCTTATATGGTCGGTACCGAG
CTCGAATTCGATATCGAAATTGAAGGAAAAAACATCATTTGTGTTTATATGTTTTTTTTT
AATTTTTCAACTTTTTTTATTTAACTATGCTAATCATATTATAAACATTTTAAAAATCAA
GTCCAACTATTTATGAATCATTGAAGAGACAACATAATTATAACAGTAAAAATAATAATA
ATAATAATAATTTTGTCTTATTGTTTCAAACATATTATGTTCCATAAATATCTAAATTTT
TAATATGGTCATAAATTCATAGGATTTAATTTATATAAAATATGGCTATTCATACTAGCC
ATTTTATGTGTGTAAAGTTTTTTTTTTCTGAACAAAAAATAAAATGTTCATAATTTTAGC
TATTTACATGCATGTGCATGCACAAAAAAAAATATGCACACAACATACACATTTTTACAG
TTATAAATACAATCAATTGGTATAAATATATAAGTAAGAAAAACGGGATCAAAGCTAGCT
TCTGTATTTCCGCTAGACAGCCATCTCCATCTGGATTCGTCCGTGCGGGACGTAGCCCAC
GACCTCAAAATCCTCGGCGGTGAAATCGTCGATTTCCTTGATGCGTTCCTTGTTGAGGAT
GTTCACAATGGGGAACGGTCTCGGTTCTCTCCGCAGCTGCTCTTTTAAAGCCTCGACATG
GTTCGTGTAGACATGCGTGTTCCCCATGAAGTGAATGAACTCCTTAGGTTTTAGGTTGCA
GACGTGTGCAACCATGAGCGTCAAAAGCGAATAGGAAGCGATGTTGAAGGGGACGCCGAG
GCCGACATCGCACGACCGCTGATACATGATGCACGACAGCTCCTTCTGGTCGTTCACGTA
GAACTGGCACAACAAGTGACAAGGCGGCAGCGCCATTTCGTCCAGCGCTGCAGGATTCCA
GGCAGTCATGAGCATGCGACGATCTGTTGGATTCGTTCTCAGCATCTGGATCACATTCTT
CAGCTGGTCGACGCCCTGCCCTGTGTAGTCTGTGTGCATGTCTTTGTATGCCGCGCCGAA
GTGTCTCCACTGGAAGCCGTAGCCCGGGCCGATGTCTCCGACCTCTCGGTGGGGGAGATT
GCGCGAATCGAGGAACTCGCGTGTCACATTCTTGTCCCAGATCTTCACGCCCTTCTCAGA
AAGATGGTTTGCGTTCGTGTCGCCGCGAATGAACCACAGCAACTCTTCGAGGACCCCTTT
CCAGAACACACGCTTTGTGGTGAGAAGTGGAAAGGCCTGATCCAGCGAGTAGCGCATAGT
GCAGCCGAATTTGGAGATGACACCAACGCCCGTTCGGTCATCCATTGTCCTTCCATTGTT
AATAATGTCGGCAATGAGATCAAGGTACTGGAACTCTTCATGGCCTCTAAAGTGAACATG
CGGAACGGCCCGAATCAGTTCCTTTTGCTCGCGTTTTTTCCGGTCTTCTTCGTCCATCCA
CGCCAACACCGGGGCAATGGCTGCGGCCGAAGAAGGAGCCTGCAACCCGTGCACGGGAGT
TGTCTCCCTCGTGGACGTCAAGGAGCTCATTGCGTTGCTCGGTTCCGCAGTGGCTGCGTC
GTCAGTCTTCCTTCTCTTCTCGAGAACCACAAAGTCGTAGGGAACCCCGTTGTCTGAGAA
GGTCTTGGAAATGAAGATGGGTCGATACGTCGCTTCATTGTCCTTCTCTCTTCCGAGCTC
CGGACAAAAGGGAACGAACACAGACTCGGCAGGAGCTGCAGCCTGCGCAGCAGTTGATTT
GTTTGAAAGAATGTCATCTCCGGGGAACGCAGGGAAGAAAACGTCGCACGGAAACTCGCG
GGCTACACGCGTGATGTACAGGTGAGAGGCAACGCCCAGAGACAGCGCTGCCTCGTACAG
TCCCGCTCCTCCCACGACAAAAATCTGGTCGACAGAATCCTTGTACTCTTCCTCCAGAAG
GCTGAGAGCTGCTGGGAGTGAAGCACAGACTCGGACGCGCTGCTGGCCTTCAGCTTGAGG
CTTCTCCGCCGCAATGTCTTCTTCTTTGAGGGAAGAGGAAACGACGATGTTCAATCTGTC
CACGAGGGGTCTAAACTTTCGAGGCATGCTTTCCCAGGTTTTCCGTCCCATGACAACGGC
GTTGAATCTCTTGCCGACTGATGGAGAGGGAAGTCCAGAGTCGCCCGTCTTTGCAAATTT
CCTGGGAAGCCACCCGTTCAGGCGACTGGCTTCTTCGGGCGTCGTTTTTGTCACACGAGA
AAAGTGTTTGAAATCTGTGGTCAAGTGGGGCCACGGGAGGCCGTTGTTGATGCCGATGCC
CCTCTTGGGGGTCATCGCGACGACCAGACACACCGGTTTATGCATTGATCCTTTGTAACA
TTTAGGTGTGTATTTATATATATAAGCAATATACAAATGAAAATACTATTATAAAACGGA
AACTCAAAAAATGTTGAAACAAATAGTCAATTTTTAAAATGAATGTGTAAATTGTTTAAG
TTTTATGACAAAAAAAGGAAAAGTGTATATAAAATAAAAATTGGAAAAAATTTATATATT
ACATGCCATATAATACCATTTATTTACTATTTATATCTTAATATAAAAAATTGTAGCAAA
AATATATAATCGTATTTTATATATTTAATATTATATAACCCCTTAAAAAAAAAAATTTAA
TTGAAAAAATTTTAATTTATGCACTTTTTTATTGTATTATATATATTCCGTTATTTTAAT
TACACATAAACTTAATAGAAAAATTAATTCGCTGCTAAATAAAAATTATAGGGCTATATA
TGATGGGATCATATTCTATCTTTCTCTTTTTCAAATYCTTTATATTTTAGAATACAAATC
GCTTTAAACAATAAAAAAGAGGTATATCCTACCCGAATTGACACACACAATAAATAATAA
ATATATAAATAATAAAAATATAAATATATAAATTATAAATATATAAATTATATATATAAA
TATATTAACATGTGTGTAATTTGTTAAAATTGATATTAAACAGCTTTGCATGGGGAAAGA
GTGGCTTTTATAAGCGAATCATATAATACAGTGTTAATTTTAATATATAAAATATAATTT
CCTTAAATTGTTATTTAACATTTTTAATTGCCTACCCARGGTAATATAATATCCAAATGA
CAATCAAAGATAAATGAAATACCGCTCCATTTTTCCTATATATCCATTTTCATTTTGTGT
AAATGTTCATAAAATTTTATCATATACTCATAAAAAACAGGGGAAAAATGAAAAAATGAA
CGTCCACCCCGTGTGAATATGCTCATTTTGTATACTATACATTTTTTTTTATATAATTTA
TATTTTCCAAATAAAAAAAATGTTTGTACATGTATATATCAATATTGTGTTATGGCATGA
TGAAAAACAATTATTTTATTCGTTTTTAAATTTGTAGAATTTCCCTAAATTATTAATGAT
TTCATATTATAATATATGTATCTATATATTTTTAATGTGTAATTCAAAATGGCGAAAAAG
AAACAAACCATACTTTATAAGCAACTCTCTTTGAGCACTTACCATAAAAAATTATACTAC
TAAATTCAAATTAAATAAAAAAATATATATTATAAAATTGGCTTTAATTATTTATAAGCA
CTTGCTTTGTTTTACTCAAAAAGTATTGGCATAAGTATAAACATAAAACCAATTTTTCAC
AAAATATATTATATGTCCATACAACTATATCCGAACYTTCAAAACACATCCAAATTTCCA
TTTACCACATGCAAGCAAAAGGATATTAAATAAAAAAAAATTATTTATATTTTCATGATC
ACTTAAATAATATTTAAAAAAAAAAGAGACCATATATAAAATGTATAATTTAAATATAGC
TAACTTATTAAAAAAAATGAATATTGAGCATATATCCATAATTTAAAACACTTCTATTTT
ATGTTATTTGATATATAAGTCGATAAATTATTGCATGTATGCATATAGACAGATTAATAT
ACACCAAAATAATAAAAATTATATATAGTTCATATATTATTCTGAATAATAAATTAGCTT
TCCTCTTAATTCACTTGGTATGTATATATATATATATATATATATATATATATATATGGA
TAAATAAATATAAGGTCTATAACAGGAAAAAATGTGTATTAACTTTTTTTTATAATTATT
TTATATTTTTTCTCCATTTATTTAAATATGTTAAAATTATTTCCCATTTTATAAACAAAA
ATTAATCCATAAAATAGCAAAATAACAAAATGACAAAATAACAAAAACAAAAAAACGCAT
TATATGAGTTCATTTTACACAATCCAAAGAAAAAGAAAATATGCTTAAAATATTCATAAC
ACACTTTTAAGCTATAAAAATATATATATTTATTTGTGTCTATATTACCAACTCAATTTA
ATAGATGTGTTATGTGATTAATTCATACACAAACATACAAAAATAAACACCATTTTTGAA
AAGATTAATTTGAAATCATATATGTTATTATTATATATTTTTATATCAATGAAATTTAAA
AAAATAAAAGGGAAATCAATGTATTAAAAATAATTATATGTTATTTTATTTCCACAATAT
TTATTTATTATTTATTGTTGACCTGCAGGCATGCAAGCTTATCGATACCGTCGACCTCGA
GGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGT
AATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACA
TACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT
TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATT
AATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCT
CGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCT
CAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTA
CGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT
CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTG
GTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAA
GTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGT
CACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTA
CATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA
GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTA
CTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCT
GAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCG
CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAAC
TCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT
GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAA
ATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTT
TTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAAT
GTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC
Restriction sites to linearize plasmid PmeI
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationThe pir3::gfp DNA construct was introduced in mutant RMgm-1281 that contains a mCherry-tagged version of pir1 and gfp-luciferase in the 230p locus (under the control of the ama1 promoter)
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1CCCCGCGGCGATAATTATAAATAATATGGTAGATTG
Additional information primer 1L4818 (SacII); PBANKA_140030 Forward
Sequence Primer 2CGGGATCCCCGATAATTCATTTTCTTCTTT
Additional information primer 2L4819 (BamHI); PBANKA_140030 Reverse
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_0500200
Gene Model P. falciparum ortholog Not available
Gene productBIR protein; PIR protein
Gene product: Alternative namePIR3
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Details of the genetic modification
Name of the tagEGFP
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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AGCTTGGGCCCCCGCGGATCGATAGATCTGTTAACAGGCCTCTGCAGCCCAGCTTAATTC
TTTTCGAGCTCTTTATGCTTAAGTTTACAATTTAATATTCATACTTTAAGTATTTTTTGT
AGTATCCTAGATATTGTGCTTTAAATGCTCACCCCTCAAAGCACCAGTAATATTTTCATC
CACTGAAATACCATTAAATTTTCAAAAAAATACTATGCATATAATGTTATACATATAAAC
ATAAAACGCCATGTAAATCAAAAAATATATAAAAATATGTATAAAAATAAATATGCACTA
AATATAAGCTAATTATGCATAAAAATTAAAGTGCCCTTTATTAACTAGTCGTAATTATTT
ATATTTCTATGTTATAAAAAAATCCTCATATAATAATATAATTAATATATGTAATGTTTT
TTTTATTTTATAATTTTAATATAAAATAATATGTAAATTAATTCAAAAAATAAATATAAT
TGTTGTGAAACAAAAAACGTAATTTTTTCATTTGCCTTCAAAATTTAAATTTATTTTAAT
ATTTCCTAAAATATATATACTTTGTGTATAAATATATAAAAATATATATTTGCTTATAAA
TAAATAAAAAATTTTATAAAACATAGGGGGATCCATGGTTGGTTCGCTAAACTGCATCGT
CGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGACCTGCCCTGGCCACCGCTCAG
GAACGAATTTAGATATTTCCAGAGAATGACCACAACCTCTTCAGTAGAAGGTAAACAGAA
TCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAA
GGGTAGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTT
TCTTTCCAGAAGTCTAGATGATGCCTTAAAACTTACTGAACAACCAGAATTAGCAAATAA
AGTAGACATGGTCTGGATAGTTGGTGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCC
AGGCCATCTTAAACTATTTGTGACAAGGATCATGCAAGACTTTGAAAGTGACACGTTTTT
TCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGAATACCCAGGTGTTCTCTCTGA
TGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGTATATGAGAAGAATGATTAAGG
ATCCCGTTTTTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTAAAAATG
TGTATGTTGTGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAAAATTAT
GAACATTTTATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTAGTATGA
ATAGCCATATTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAATTTAGAT
ATTTATGGAACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTATTATTT
TTACTGTTATAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGATTTTTAA
AATGTTTATAATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACA
TATAAACACAAATGATGTTTTTTCCTTCAATTTCGATGGGTACCCAGCTTTTGTTCCCTT
TAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAT
TGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGG
GGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAG
TCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT
TTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG
GATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAG
GCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA
CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT
GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCC
TTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCG
GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC
TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA
CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG
TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCT
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACC
ACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGA
TCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA
CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT
TAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTAC
CAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTT
GCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGT
GCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAG
CCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCT
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT
GTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGC
TCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTT
AGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATG
GTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTG
ACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCT
TGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATC
ATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGT
TCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTT
TCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGG
AAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTAT
TGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCG
CGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCG
CGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCC
CTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGA
GTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCG
ATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAG
CACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGA
ACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTG
TAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCG
CGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTT
CGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGC
CAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACT
CACTATAGGGCGAATTGGAGCTCCACCGCGGCGTATATGCATTAATAACTATAACAATAG
AATATATAAAATAAAAATGAACTGTGCAAAACATTTAGCGAATATTTATCTAAATTTATA
TAGTAAAAGAGAAAATATATCTTATCTCTCTTCTCTTAAAATGCAATATAACAACCTAAT
TAAACATAACTTTATATTATACTTTAAAATTTGCATCAATACTTATTTATGTGTTAATAT
TTACTAAGCATTTTTTTAAAAATAATATGCATTCAAATACTTGTTTAAGCTTATAAATAC
TGGTCCAGTGCTAATTGTTATTTTAAATAATGTAAAAGATGGGAAAATATATAATAAAAT
TATCCAACAGAGAATACTTCTAAATTGGGATATGCAGGAATAAAAATAAAGTTATTTAAC
GCATTAAAATTATTTTTTAATTTATCTATATTAAATACAAATAATATAAATTTAAATAAT
TTGTATAGAAAATGAGGCATGTAACTAAATTTGAAAATGCTATTATTTTAGAAAAAACTA
TAATATATATTATAAGAAACAAGTATACACAAATTTAATATATTTAAAAAATCACTATTT
ATATATTTTAAGAATTCTAATAATAATAACTTTCTTAATTTTTTGTATTTGTGTAGTATT
TGAGTTTTATGACGTTGTTTGTGTTCTATATTAAAGCATATGTATAAGTATATATTTGTT
AAATTCATTATAAAAGTATATCTATTTTTATAATAAAGTTATACCAAGTGTTAGTAAGAA
TAGTATTTTTTGTATAAAATTCATCATATATACATATGGCAAAAGTGTATAAGCAACCTT
CTATTTAAGGTAATTTAGCTAACTGCCATAAAATAAGTATAATATAATTTTAGTAATACT
AATGTATTATTATTTTACATGAAGTTAAAATTAAGAATAATATGTCTAACGAAAGATAAT
TTATATGTAAAGATCTTGAGTAAATATACCATATATTCTATGCAATATATATAAACAACA
TCACCCCGCATAATCTCCAAATTATAACAGAATTTCATTATAATGTCTTTTAGAGTGGTA
TATACATTTTTTCTTATATTATTCGTATGTTGCATTCCTACAAATTATATTAATTTTAAT
TTTAGTAACATTTATATTAATACATTTTTTGTTTAATTCGTAAAATATATTCGTAGTGTT
ATATAATTAGTGAGATTGATAATTTTGCTAATGATCCGAAAAACCAGGGAGGACATAATT
CTATTGGTTATTTTAAGTATTATTGCCCTGATAATAACTGTGATACTGATGTCAAAAAAG
TTATTTCTACTTTTATAGCATTAGTAACTTTATTTAATGGTATTAATCATAATGAAAAAT
TAGAGAGTGATAAAATTGCTGAATATGCTATTTTATGGTTAAGTTATAAACTAAATCAAA
AAACACAAAATGGAAACACCAAATTATACGATTTTTATACTGAACATATAAACACAAATA
GTAAATATAATGAGCATATAACTAATGATTTTAATATTAATAAGAGTGTTATAGAAAATA
AAATAAAATTGATGAATATGAATATTAAAGATATATCTAAATTTTATGATGCATTTAAAA
AATTATGTGAAATGTATACTGAATTTGATGACGACAATAAAAATTGCACAAACTGTTCGG
GAAAAGCTAAAGAATTTGTTGAAAAATATAAAGACCTTAACAAAGATTATAATAACACTA
ATAGCAGTTCCTATAATAAAATGTTGTCTACTTTATCAACTGATTATAATAATTTAAAAA
ATAAATGTAAAAATGTTCAAAATAGCAAGTTTCCACTCCTTCCAGAGATAAAAACAACAC
AAATTTCTGTAAAAAGTTCTGAACAAACTTCTGCACAAACATCTGAAGTTGCATCATCAA
GGTCGTCGATAGGAAACAACTTATTTACAGTTTTATCGGTATTTGGTGCAATAGCATTAT
TTTTAGGAATTGGATATAAGGTAAATAATAAGGAATTAAAAAAAAATACTATATATATGT
AAACATTAACAAACAACCGTACGCTTCTTAACATTTTATATTAGCATTCGTTATTTGGAT
TTCGGAGAAAAGTTCAAAAAAATTTAAGAAAAAAGCTAAAATCATTAAGAAGAAAATGGA
TAATTAGTATAGGATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGGGCGAGGAGCTGT
TCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA
GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCT
GCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCG
TGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCA
TGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGA
CCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCA
TCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCC
ACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCC
GCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCA
TCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGA
GCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCG
GGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGAATTATTA
ATATATATGAATATATATACATCGTTGTATGCGTAATATACGTGTAACTGTTTTTTTTAA
TTTTTATATTAACTTTTCCTTATACATTTTAATGCCATTAACTAATAATATTTTACTTGA
AAAAATTCTAAATTTTTATAACTCTAATATAAAATCTGTGAATAATAAAAAATTAGCATA
AAGTACGAATTAAACATCGAATATTTGTTTTAAAGGGGCACACATTATGTGCATATAATT
TTATTCTTTGAATTTAATTTTTTGCTTATAATTAGATTAAATTAATATAATTTTTTTATT
GAAAATAAAAACTTATTTTAAAAAATTAACAAATGGAAGTTAATGAAAAATACGAAAAAA
GTAAAGGGTTAATTCTTATATGGTCGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA
TA
Restriction sites to linearize plasmid BsiWI
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedureWR99210
Selection (negative) procedureNo
Additional remarks genetic modificationThe pir3::gfp DNA construct was introduced in mutant RMgm-1281 that contains a mCherry-tagged version of pir1 and gfp-luciferase in the 230p locus (under the control of the ama1 promoter)
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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  Transgene: Mutant parasite expressing a transgene
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Type and details of transgene
Is the transgene Plasmodium derived Transgene: not Plasmodium
Transgene nameA fusion of GFP (gfp-mu3) and Luciferase Firefly (LucIAV)
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitegfp (FACS)
Promoter of the selectable markerama-1
Selection (positive) procedureFACS (flowsorting)
Selection (negative) procedureNo
Additional remarks genetic modificationThe GFP-Luciferase gene (1 copy) has been inserted into the 230p locus (PB000214.00.0) by double cross-over integration.
Additional remarks selection procedureThis reporter mutant expressing GFP-Luciferase does not contain a drug-selectable marker. This mutant has been selected by FACS sorting after transfection based on GFP fluorescence.
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Other details transgene
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Promoter
Gene Model of Parasite PBANKA_0915000
Gene Model P. falciparum ortholog PF3D7_1133400
Gene productapical membrane antigen 1
Gene product: Alternative nameama-1
Primer information details of the primers used for amplification of the promoter sequence  Click to view information
Primer information details of the primers used for amplification of the promoter sequence  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
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3'-UTR
Gene Model of Parasite PBANKA_0719300
Gene productbifunctional dihydrofolate reductase-thymidylate synthase, putative
Gene product: Alternative namedhfr/ts
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to view information
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Insertion/Replacement locus
Replacement / InsertionReplacement locus
Gene Model of Parasite PBANKA_0306000
Gene product6-cysteine protein
Gene product: Alternative name230p
Primer information details of the primers used for amplification of the target sequences  Click to view information
Primer information details of the primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: StudioDrie StudioDrie; S. Mededovic and A. van Wigcheren