SummaryRMgm-1217
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Other |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 25732065 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Gomes AR; Billker O |
Name Group/Department | Wellcome Trust Sanger Institute |
Name Institute | Wellcome Trust Sanger Institute |
City | Hinxton Cambridge |
Country | UK |
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Name of the mutant parasite | |
RMgm number | RMgm-1217 |
Principal name | - |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | No |
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Phenotype | |
Asexual blood stage | Reduced fitness (growth rate) |
Gametocyte/Gamete | Not tested |
Fertilization and ookinete | Not tested |
Oocyst | Not tested |
Sporozoite | Not tested |
Liver stage | Not tested |
Additional remarks phenotype | Successful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants. Evidence is provided for reduced fitness (growth rate) of blood stages In this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest. To analyze growth curves derived from barcode counting two parameters were considered: (1) the relative abundance of each barcode within the pool, and (2) the relative fitness of each mutant, i.e., the rate at which its abundance changed each day (i.e. growth rate). |
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Details of the target gene | |
Gene Model of Rodent Parasite | PBANKA_1445600 |
Gene Model P. falciparum ortholog | PF3D7_1230900 |
Gene product | serine/threonine protein kinase RIO1, putative |
Gene product: Alternative name | RIO1 |
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Description | |
Short description of the modification | Successful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants |
Description | In this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest. In addition the vectors contain gene-specific molecular barcodes. Co-transfecting multiple gene-deletion vectors in the same electroporation reproducibly generates complex pools of barcoded P. berghei mutants. In this study parasites were (mainly) transfected with a pool of vectors targeting protein kinase genes for deletion. Unsuccessful gene disruption/deletion or successful gene disruption/deletion is determined by the absence or presence of the barcode in the population (as determined by barcode-specific PCR analysis). The gene was targeted by PlasmoGEM vector PbGEM-065291 |
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