RMgmDB - Rodent Malaria genetically modified Parasites
SummaryRMgm-1192
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Last modified: 21 March 2015, 19:51
*RMgm-1192| Successful modification | The gene/parasite could not be changed/generated by the genetic modification. |
| The following genetic modifications were attempted | Other |
| Number of attempts to introduce the genetic modification | 2 |
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 25732065 |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone | Not applicable |
| Other information parent line | |
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| Attempts to generate the mutant parasite were performed by | |
| Name PI/Researcher | Gomes AR; Billker O |
| Name Group/Department | Wellcome Trust Sanger Institute |
| Name Institute | Wellcome Trust Sanger Institute |
| City | Hinxton Cambridge |
| Country | UK |
Other: Mutant parasite with another genetic modification| top of page | |
| Details of the target gene | |
| Gene Model of Rodent Parasite | PBANKA_1126900 |
| Gene Model P. falciparum ortholog | PF3D7_0628200 |
| Gene product | protein kinase PK4 | eukaryotic translation initiation factor 2-alpha kinase |
| Gene product: Alternative name | PK4; PERK |
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| Description | |
| Short description of the modification | Unsuccessful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants |
| Description | In this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest. In addition the vectors contain gene-specific molecular barcodes. Co-transfecting multiple gene-deletion vectors in the same electroporation reproducibly generates complex pools of barcoded P. berghei mutants. In this study parasites were (mainly) transfected with a pool of vectors targeting protein kinase genes for deletion. Unsccessful gene disruption/deletion or successful gene disruption/deletion is determined by the absence or presence of the barcode in the population (as determined by barcode-specific PCR analysis). The gene was targeted by PlasmoGEM vector PbGEM-099789 |
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