RMgmDB - Rodent Malaria genetically modified Parasites - P. berghei

RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-1187

Malaria parasite	P. berghei
Genotype
Genetic modification not successful
Other modification	Gene model (rodent): PBANKA_1443000; Gene model (P.falciparum): PF3D7_1228300; Gene product: NIMA related kinase 1 (NEK1) Details modification: Unsuccessful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants
Phenotype	No phenotype has been described

Last modified: 21 March 2015, 19:40

*RMgm-1187

Successful modification	The gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted	Other
Number of attempts to introduce the genetic modification	2
Reference (PubMed-PMID number)	Reference 1 (PMID number) : 25732065
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Parent parasite used to introduce the genetic modification
Rodent Malaria Parasite	P. berghei
Parent strain/line	P. berghei ANKA
Name parent line/clone	Not applicable
Other information parent line
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Attempts to generate the mutant parasite were performed by
Name PI/Researcher	Gomes AR; Billker O
Name Group/Department	Wellcome Trust Sanger Institute
Name Institute	Wellcome Trust Sanger Institute
City	Hinxton Cambridge
Country	UK

Other: Mutant parasite with another genetic modification

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Details of the target gene
Gene Model of Rodent Parasite	PBANKA_1443000
Gene Model P. falciparum ortholog	PF3D7_1228300
Gene product	NIMA related kinase 1
Gene product: Alternative name	NEK1
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Description
Short description of the modification	Unsuccessful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants
Description	In this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest. In addition the vectors contain gene-specific molecular barcodes. Co-transfecting multiple gene-deletion vectors in the same electroporation reproducibly generates complex pools of barcoded P. berghei mutants. In this study parasites were (mainly) transfected with a pool of vectors targeting protein kinase genes for deletion. Unsccessful gene disruption/deletion or successful gene disruption/deletion is determined by the absence or presence of the barcode in the population (as determined by barcode-specific PCR analysis). The gene was targeted by PlasmoGEM vector ??
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