RMgmDB - Rodent Malaria genetically modified Parasites
SummaryRMgm-1131
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Last modified: 28 September 2014, 20:08
*RMgm-1131| Successful modification | The gene/parasite could not be changed/generated by the genetic modification. |
| The following genetic modifications were attempted | Gene disruption |
| Number of attempts to introduce the genetic modification | Unknown |
| Reference (PubMed-PMID number) | Not published (yet) |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | not reported |
| Name parent line/clone | Not applicable |
| Other information parent line | |
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| Attempts to generate the mutant parasite were performed by | |
| Name PI/Researcher | Sanders, NG; Sullivan, DJ |
| Name Group/Department | Johns Hopkins Bloomberg School of Public Health |
| Name Institute | Johns Hopkins Bloomberg School of Public Health |
| City | Baltimore, Maryland |
| Country | US |
Disrupted: Mutant parasite with a disrupted gene| top of page | |||||||||||||||||||||||||
| Details of the target gene | |||||||||||||||||||||||||
| Gene Model of Rodent Parasite | PBANKA_1319100 | ||||||||||||||||||||||||
| Gene Model P. falciparum ortholog | PF3D7_1455400 | ||||||||||||||||||||||||
| Gene product | hemolysin III | ||||||||||||||||||||||||
| Gene product: Alternative name | HlyIII | ||||||||||||||||||||||||
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| Details of the genetic modification | |||||||||||||||||||||||||
| Inducable system used | No | ||||||||||||||||||||||||
| Additional remarks inducable system | |||||||||||||||||||||||||
| Type of plasmid/construct used | not known | ||||||||||||||||||||||||
| PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
| Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
| Plasmid/construct map | |||||||||||||||||||||||||
| Plasmid/construct sequence | |||||||||||||||||||||||||
| Restriction sites to linearize plasmid | |||||||||||||||||||||||||
| Partial or complete disruption of the gene | Unknown | ||||||||||||||||||||||||
| Additional remarks partial/complete disruption | This mutant has been reported on the 2014 (25th) Annual Molecular Parasitology Meeting, Woodshole. In the abstract no data is provided on the details of the construct used in the attempts to knock-out this gene. When these unsuccessful attempts are published, additional information will be added to this mutant-entry. | ||||||||||||||||||||||||
| Selectable marker used to select the mutant parasite | not known | ||||||||||||||||||||||||
| Promoter of the selectable marker | not known | ||||||||||||||||||||||||
| Selection (positive) procedure | not known | ||||||||||||||||||||||||
| Selection (negative) procedure | not known | ||||||||||||||||||||||||
| Additional remarks genetic modification | This mutant has been reported on the 2014 (25th) Annual Molecular Parasitology Meeting, Woodshole. In the abstract no data is provided on the details of the construct used in the attempts to knock-out this gene. When these unsuccessful attempts are published, additional information will be added to this mutant-entry. The unsuccessful attempts to knock-out this gene indicates that the protein is essential for blood stage development/multiplication. From the Abstract: Previous work in our lab demonstrated that PfHlyIII is a pore-forming protein that lyses uninfected red cells in a temperature-dependent manner and is inhibited by addition of polyethylene glycol of increasing molecular weight, suggesting a pore diameter of approximately 3 nm. Transfection of P. falciparum Dd2attB with GFP C-terminally tagged HlyIII suggests localization in the digestive vacuole of the parasite. Our current studies demonstrate Xenopus laevis oocytes expressing recombinant PfHlyIII swell and rupture over time, with rupture inhibited by increasing molecular weight polyethylene glycol. Attempts to knockout the gene in P. falciparum and P. berghei were unsuccessful, suggesting but not proving an essential role in the parasite. See also RMgm-824 for independent unsuccessful attempts to knock-out this gene in P. berghei | ||||||||||||||||||||||||
| Additional remarks selection procedure | |||||||||||||||||||||||||
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Primer information: Primers used for amplification of the target sequences
 Primer information: Primers used for amplification of the target sequences
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