RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-105

Malaria parasite	P. berghei
Genotype
Disrupted	Gene model (rodent): PBANKA_0934600; Gene model (P.falciparum): PF3D7_1113000; Gene product: conserved Plasmodium protein, unknown function
Phenotype	No phenotype has been described

Last modified: 9 July 2010, 14:47

*RMgm-105

Successful modification	The parasite was generated by the genetic modification
The mutant contains the following genetic modification(s)	Gene disruption
Reference (PubMed-PMID number)	Reference 1 (PMID number) : 18974882
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria Parasite	P. berghei
Parent strain/line	P. berghei ANKA
Name parent line/clone	P. berghei ANKA 507cl1 (RMgm-7)
Other information parent line	P.berghei ANKA 507cl1 (RMgm-7) is a reference ANKA mutant line which expresses GFP under control of a constitutive promoter (PubMed: PMID: 16242190).
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The mutant parasite was generated by
Name PI/Researcher	E. Lasonder, C.J. Janse, H.G. Stunnenberg
Name Group/Department	Department of Molecular Biology
Name Institute	NCMLS, Radboud University Nijmegen
City	Nijmegen
Country	The Netherlands
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Name of the mutant parasite
RMgm number	RMgm-105
Principal name	800; 836
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modification	No
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Phenotype
Asexual blood stage	Not different from wild type
Gametocyte/Gamete	Not different from wild type
Fertilization and ookinete	Not different from wild type
Oocyst	Not different from wild type
Sporozoite	Not different from wild type
Liver stage	Not different from wild type
Additional remarks phenotype	Mutant/mutation The mutant lacks expression of PBANKA_093460 Protein (function) Unknown. The protein was detected in a proteome analysis of P. falciparum oocysts and sporozoites. It was highly abundant in oocyst-derived sporozoites and not detected in salivary gland sporozoites. It contains a transmembrane region. Phenotype The phenotype has not been analysed in detail and the mutants have not been cloned after transfection. Sporozoite production was not significantly different from wild type and sporozoites were infectious to C57BL/6 mice

Disrupted: Mutant parasite with a disrupted gene

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Details of the target gene

Gene Model of Rodent Parasite PBANKA_0934600

Gene Model P. falciparum ortholog PF3D7_1113000

Gene product conserved Plasmodium protein, unknown function

Gene product: Alternative name

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Details of the genetic modification

Inducable system used No

Additional remarks inducable system

Type of plasmid/construct used Plasmid double cross-over

PlasmoGEM (Sanger) construct/vector used No

Modified PlasmoGEM construct/vector used No

Plasmid/construct map

Plasmid/construct sequence

GCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTA

ACCCTCACTAAAGGGAACAAAAGCTGGTACCAATACATTATTAATTGGAATGCAAACTAT

TTTATACTAACTTTTTTAAAATGATTTTATCGAAATTTTTTTAGTATTATATAATTACAT

ATTTATTAACTTTTTTTCATTTTAGACTTATGCTCCTTAATATGTATATTTACACATATA

TGTATGTATGGCTTAGTCATAGTATTGACACAAAAATTGTGAAGGGCAATGTTTAGTTAT

TTGTAAAACATTATTCTTCTCCTTTTTTTTGAATGGTGGTATTGTTTTGTTTCATTACTT

ATTTTTATTAACTTCATATATAGTTATAATTTTGTTCTTTCCTCATATTTATCACATAAA

TTTAAATACACCATTAGAAATATAATTATTTAATTAAAAAACATAAAAATACTTTTAAAA

AAATGTTCTCAAATAATAAAAGAACAATCATTTGTGAGATACTATTTTTTTTCGCTTATT

TTCTAAAAAATGGTAATAAATACTTGCTGTCTTAATAATATTCGATTGATAAATTATGGA

AATATTTTAAGATTTTACGAAAATATATCTCTGTACAATCTACCTAGTGCAATAATCCAT

AACAAATATACATGTAAAGTCATATGCATGTATCGATTAATTCACTCCGTTTTATTGTTT

GCCTTATTTGCCAGGTGGCCAATGTAGCGATATATTTCCAAGGAGTCTGTTTTTAAAAAG

GGTAATAACGAAGAGAAAAGTTGTAATTGAATAAGTTAATATTAGCGCAAAATTAAGAAA

AAGACACTATTATTACAATATAAGATCATCATGTTATGAGCCCATAAATAAAACACAAAT

TATTGTCTTCCTTTAGAACCACCCTTCCAATCTAGTGCATATTTTTAAAGATACCAAAGG

TTAGAGGAACTCAATATATATGTTACTATTTTTTAAATCCTACCTTATTCTTACATCTTA

GTTTTTTTGTATCTACCACGTGGAATACCATTGCTTTGTTCATTTACTTTTCCTATTTAT

TGAAAGTACTAATATTTCAATTGATGCCTTATTTTTCTATCACTACTCGCATATTCTGAT

TATACAAGCTTGCATGCCTGCAGGTCAACAATAAATAATAAATAAATATTGTGGAAATAA

AATAACATATAATTATTTTTAATACATTGATTTCCCTTTTATTTTTTTAAATTTCATTGA

TATAAAAATATATAATAATAACATATATGATTTCAAATTAATCTTTTCAAAAATGGTGTT

TATTTTTGTATGTTTGTGTATGAATTAATCACATAACACATCTATTAAATTGAGTTGGTA

ATATAGACACAAATAAATATATATATTTTTATAGCTTAAAAGTGTGTTATGAATATTTTA

AGCATATTTTCTTTTTCTTTGGATTGTGTAAAATGAACTCATATAATGCGTTTTTTTGTT

TTTGTTATTTTGTCATTTTGTTATTTTGCTATTTTATGGATTAATTTTTGTTTATAAAAT

GGGAAATAATTTTAACATATTTAAATAAATGGAGAAAAAATATAAAATAATTATAAAAAA

AAGTTAATACACATTTTTTCCTGTTATAGACCTTATATTTATTTATCCATATATATATAT

ATATATATATATATATATATATACATACCAAGTGAATTAAGAGGAAAGCTAATTTATTAT

TCAGAATAATATATGAACTATATATAATTTTTATTATTTTGGTGTATATTAATCTGTCTA

TATGCATACATGCAATAATTTATCGACTTATATATCAAATAACATAAAATAGAAGTGTTT

TAAATTATGGATATATGCTCAATATTCATTTTTTTTAATAAGTTAGCTATATTTAAATTA

TACATTTTATATATGGTCTCTTTTTTTTTTAAATATTATTTAAGTGATCATGAAAATATA

AATAATTTTTTTTTATTTAATATCCTTTTGCTTGCATGTGGTAAATGGAAATTTGGATGT

GTTTTGAARGTTCGGATATAGTTGTATGGACATATAATATATTTTGTGAAAAATTGGTTT

TATGTTTATACTTATGCCAATACTTTTTGAGTAAAACAAAGCAAGTGCTTATAAATAATT

AAAGCCAATTTTATAATATATATTTTTTTATTTAATTTGAATTTAGTAGTATAATTTTTT

ATGGTAAGTGCTCAAAGAGAGTTGCTTATAAAGTATGGTTTGTTTCTTTTTCGCCATTTT

GAATTACACATTAAAAATATATAGATACATATATTATAATATGAAATCATTAATAATTTA

GGGAAATTCTACAAATTTAAAAACGAATAAAATAATTGTTTTTCATCATGCCATAACACA

ATATTGATATATACATGTACAAACATTTTTTTTATTTGGAAAATATAAATTATATAAAAA

AAAATGTATAGTATACAAAATGAGCATATTCACACGGGGTGGACGTTCATTTTTTCATTT

TTCCCCTGTTTTTTATGAGTATATGATAAAATTTTATGAACATTTACACAAAATGAAAAT

GGATATATAGGAAAAATGGAGCGGTATTTCATTTATCTTTGATTGTCATTTGGATATTAT

ATTACCYTGGGTAGGCAATTAAAAATGTTAAATAACAATTTAAGGAAATTATATTTTATA

TATTAAAATTAACACTGTATTATATGATTCGCTTATAAAAGCCACTCTTTCCCCATGCAA

AGCTGTTTAATATCAATTTTAACAAATTACACACATGTTAATATATTTATATATATAATT

TATATATTTATAATTTATATATTTATATTTTTATTATTTATATATTTATTATTTATTGTG

TGTGTCAATTCGGGTAGGATATACCTCTTTTTTATTGTTTAAAGCGATTTGTATTCTAAA

ATATAAAGRATTTGAAAAAGAGAAAGATAGAATATGATCCCATCATATATAGCCCTATAA

TTTTTATTTAGCAGCGAATTAATTTTTCTATTAAGTTTATGTGTAATTAAAATAACGGAA

TATATATAATACAATAAAAAAGTGCATAAATTAAAATTTTTTCAATTAAATTTTTTTTTT

TAAGGGGTTATATAATATTAAATATATAAAATACGATTATATATTTTTGCTACAATTTTT

TATATTAAGATATAAATAGTAAATAAATGGTATTATATGGCATGTAATATATAAATTTTT

TCCAATTTTTATTTTATATACACTTTTCCTTTTTTTGTCATAAAACTTAAACAATTTACA

CATTCATTTTAAAAATTGACTATTTGTTTCAACATTTTTTGAGTTTCCGTTTTATAATAG

TATTTTCATTTGTATATTGCTTATATATATAAATACACACCTAAATGTTACAAAGGATCA

ATGCATAAACCGGTGTGTCTGGTCGTCGCGATGACCCCCAAGAGGGGCATCGGCATCAAC

AACGGCCTCCCGTGGCCCCACTTGACCACAGATTTCAAACACTTTTCTCGTGTGACAAAA

ACGACGCCCGAAGAAGCCAGTCGCCTGAACGGGTGGCTTCCCAGGAAATTTGCAAAGACG

GGCGACTCTGGACTTCCCTCTCCATCAGTCGGCAAGAGATTCAACGCCGTTGTCATGGGA

CGGAAAACCTGGGAAAGCATGCCTCGAAAGTTTAGACCCCTCGTGGACAGATTGAACATC

GTCGTTTCCTCTTCCCTCAAAGAAGAAGACATTGCGGCGGAGAAGCCTCAAGCTGAAGGC

CAGCAGCGCGTCCGAGTCTGTGCTTCACTCCCAGCAGCTCTCAGCCTTCTGGAGGAAGAG

TACAAGGATTCTGTCGACCAGATTTTTGTCGTGGGAGGAGCGGGACTGTACGAGGCAGCG

CTGTCTCTGGGCGTTGCCTCTCACCTGTACATCACGCGTGTAGCCCGCGAGTTTCCGTGC

GACGTTTTCTTCCCTGCGTTCCCCGGAGATGACATTCTTTCAAACAAATCAACTGCTGCG

CAGGCTGCAGCTCCTGCCGAGTCTGTGTTCGTTCCCTTTTGTCCGGAGCTCGGAAGAGAG

AAGGACAATGAAGCGACGTATCGACCCATCTTCATTTCCAAGACCTTCTCAGACAACGGG

GTTCCCTACGACTTTGTGGTTCTCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCG

GAACCGAGCAACGCAATGAGCTCCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGG

TTGCAGGCTCCTTCTTCGGCCGCAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAA

GACCGGAAAAAACGCGAGCAAAAGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGA

GGCCATGAAGAGTTCCAGTACCTTGATCTCATTGCCGACATTATTAACAATGGAAGGACA

ATGGATGACCGAACGGGCGTTGGTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCG

CTGGATCAGGCCTTTCCACTTCTCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAA

GAGTTGCTGTGGTTCATTCGCGGCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTG

AAGATCTGGGACAAGAATGTGACACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGA

GAGGTCGGAGACATCGGCCCGGGCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATAC

AAAGACATGCACACAGACTACACAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAG

ATGCTGAGAACGAATCCAACAGATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCG

CTGGACGAAATGGCGCTGCCGCCTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAG

AAGGAGCTGTCGTGCATCATGTATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTC

AACATCGCTTCCTATTCGCTTTTGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCT

AAGGAGTTCATTCACTTCATGGGGAACACGCATGTCTACACGAACCATGTCGAGGCTTTA

AAAGAGCAGCTGCGGAGAGAACCGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAA

CGCATCAAGGAAATCGACGATTTCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCG

CACGGACGAATCCAGATGGAGATGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCC

CGTTTTTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTAAAAATGTGTA

TGTTGTGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAAAATTATGAAC

ATTTTATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTAGTATGAATAG

CCATATTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAATTTAGATATTT

ATGGAACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTATTATTTTTAC

TGTTATAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGATTTTTAAAATG

TTTATAATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATA

AACACAAATGATGTTTTTTCCTTCAATTTCGATATCGAATTCAAACAGATATTAATAGTA

TTCATAAAATGATTGACGATTTAATAAAACATAAAGATGTTGGATCTCTAAAAACTAGTA

TATTAGAAAATTTGATTGATTTAAATATATTAAATAATAGAAATAATAGGGTTCGAGATA

ATTATTTGAATATATTACATTTAGAAATATTTTCTTTATTTTCTTTTGGGCCATATCATA

ATATATATAATTTATTAGACGAATTATTTTTATATGATAATAAATTATTAAACTGGTTCA

AAAGATGGATAATATTAAATAAGAATACATATTATAATGATAATAATAATTTTAATCAAT

TGCTATTTATATCTTCTATAAAATATATGATAAAAACATTGAGTATGAAAATTAGTAAAA

AAACATTAACAAAAAAATACTTCAAATATATACCTAAATTATACAACAAAAAAAGAGCCA

GAAGTATAGACTTAAGGTTTGAAGAAATTAAACCAAATTCAATTTTAAAAAATGAAATTG

ATTACAAAAAAGAAAATTACGATAGTGATATGGGTAAAATTTTTTTAAATCGTGTCAAAG

AATATAAAATTAGTTCTAAACTTCGAGATAAAATTTTAGAAGAATTAGACAAATATAAAT

TCTCGATATCATTAGACCGTTATAAACAAGATTATTATTTTTTATCTTTAGTAGGTTTAG

GTTATTCATATATTAACAAATTTAGTGAATTAAAGTTAGGCCTATATAAATCTGTTAATA

TTATATTAAAAAAAATGTATAATATATTTTATGTTACTGAATTAATAGAAACTGTAAATG

TATTTGTAAATGTGATTGTAAATATTTATAAAAACATATTTTATAATAATAGTAAATATA

TAATTGAAAATGGAAAAATAAAATTAAGTAAAGAAAAAAAAGAAGAATTAGCTAAATTTA

TCTTATTAGAAGAAAATATAAACATCGATTGTCTACATAATCACATTTACAAAGGGCATA

AGTTTTTTACTCATGATAATTTTGATGATACAAATTATTCAGCGGCCGCCACCGCGGTGG

AGCTCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAA

CGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCT

TTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC

AGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTG

GTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTC

TTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTC

CCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGT

GATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAG

TCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCG

GTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAG

CTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTG

GCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAA

ATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGA

AGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC

TTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGG

GTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTC

GCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTAT

TATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATG

ACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAG

AATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAA

CGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC

GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCA

CGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTC

TAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTC

TGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTG

GGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA

TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAG

GTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA

TTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATC

TCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAA

AGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA

AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC

CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGT

AGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC

TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAC

GATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCA

GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG

CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG

GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT

TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAT

GGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT

GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG

CGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCA

GCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGA

GTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGT

GTGGAATTGTGA

Restriction sites to linearize plasmid

Partial or complete disruption of the gene Partial

Additional remarks partial/complete disruption See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).

Selectable marker used to select the mutant parasite tgdhfr

Promoter of the selectable marker pbdhfr

Selection (positive) procedure pyrimethamine

Selection (negative) procedure No

Additional remarks genetic modification The genetic modification results in the disruption of the following gene models PB001073.00.0, PB000529.02.0 and PB000863.00.0 (= PF11_0528).
See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).

Additional remarks selection procedure

Primer information: Primers used for amplification of the target sequences Click to view information

Primer information: Primers used for amplification of the target sequences Click to hide information

Sequence Primer 1	AAAGGTACCAATACATTATTAATTGG
Additional information primer 1	L2644 (Asp718); 5'
Sequence Primer 2	AAAAAGCTTGTATAATCAGAATATGCG
Additional information primer 2	L2645 (HindIII); 5'
Sequence Primer 3	AAAGAATTCAAACAGATATTAATAG
Additional information primer 3	L2646 (EcoRI); 3'
Sequence Primer 4	AAAGCGGCCGCTGAATAATTTGTATCATC
Additional information primer 4	L2647 (NotI); 3'
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6

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