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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_0934600
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Gene Model P. falciparum ortholog |
PF3D7_1113000
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Gene product | conserved Plasmodium protein, unknown function |
Gene product: Alternative name | |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct used | Plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
GCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTA
ACCCTCACTAAAGGGAACAAAAGCTGGTACCAATACATTATTAATTGGAATGCAAACTAT
TTTATACTAACTTTTTTAAAATGATTTTATCGAAATTTTTTTAGTATTATATAATTACAT
ATTTATTAACTTTTTTTCATTTTAGACTTATGCTCCTTAATATGTATATTTACACATATA
TGTATGTATGGCTTAGTCATAGTATTGACACAAAAATTGTGAAGGGCAATGTTTAGTTAT
TTGTAAAACATTATTCTTCTCCTTTTTTTTGAATGGTGGTATTGTTTTGTTTCATTACTT
ATTTTTATTAACTTCATATATAGTTATAATTTTGTTCTTTCCTCATATTTATCACATAAA
TTTAAATACACCATTAGAAATATAATTATTTAATTAAAAAACATAAAAATACTTTTAAAA
AAATGTTCTCAAATAATAAAAGAACAATCATTTGTGAGATACTATTTTTTTTCGCTTATT
TTCTAAAAAATGGTAATAAATACTTGCTGTCTTAATAATATTCGATTGATAAATTATGGA
AATATTTTAAGATTTTACGAAAATATATCTCTGTACAATCTACCTAGTGCAATAATCCAT
AACAAATATACATGTAAAGTCATATGCATGTATCGATTAATTCACTCCGTTTTATTGTTT
GCCTTATTTGCCAGGTGGCCAATGTAGCGATATATTTCCAAGGAGTCTGTTTTTAAAAAG
GGTAATAACGAAGAGAAAAGTTGTAATTGAATAAGTTAATATTAGCGCAAAATTAAGAAA
AAGACACTATTATTACAATATAAGATCATCATGTTATGAGCCCATAAATAAAACACAAAT
TATTGTCTTCCTTTAGAACCACCCTTCCAATCTAGTGCATATTTTTAAAGATACCAAAGG
TTAGAGGAACTCAATATATATGTTACTATTTTTTAAATCCTACCTTATTCTTACATCTTA
GTTTTTTTGTATCTACCACGTGGAATACCATTGCTTTGTTCATTTACTTTTCCTATTTAT
TGAAAGTACTAATATTTCAATTGATGCCTTATTTTTCTATCACTACTCGCATATTCTGAT
TATACAAGCTTGCATGCCTGCAGGTCAACAATAAATAATAAATAAATATTGTGGAAATAA
AATAACATATAATTATTTTTAATACATTGATTTCCCTTTTATTTTTTTAAATTTCATTGA
TATAAAAATATATAATAATAACATATATGATTTCAAATTAATCTTTTCAAAAATGGTGTT
TATTTTTGTATGTTTGTGTATGAATTAATCACATAACACATCTATTAAATTGAGTTGGTA
ATATAGACACAAATAAATATATATATTTTTATAGCTTAAAAGTGTGTTATGAATATTTTA
AGCATATTTTCTTTTTCTTTGGATTGTGTAAAATGAACTCATATAATGCGTTTTTTTGTT
TTTGTTATTTTGTCATTTTGTTATTTTGCTATTTTATGGATTAATTTTTGTTTATAAAAT
GGGAAATAATTTTAACATATTTAAATAAATGGAGAAAAAATATAAAATAATTATAAAAAA
AAGTTAATACACATTTTTTCCTGTTATAGACCTTATATTTATTTATCCATATATATATAT
ATATATATATATATATATATATACATACCAAGTGAATTAAGAGGAAAGCTAATTTATTAT
TCAGAATAATATATGAACTATATATAATTTTTATTATTTTGGTGTATATTAATCTGTCTA
TATGCATACATGCAATAATTTATCGACTTATATATCAAATAACATAAAATAGAAGTGTTT
TAAATTATGGATATATGCTCAATATTCATTTTTTTTAATAAGTTAGCTATATTTAAATTA
TACATTTTATATATGGTCTCTTTTTTTTTTAAATATTATTTAAGTGATCATGAAAATATA
AATAATTTTTTTTTATTTAATATCCTTTTGCTTGCATGTGGTAAATGGAAATTTGGATGT
GTTTTGAARGTTCGGATATAGTTGTATGGACATATAATATATTTTGTGAAAAATTGGTTT
TATGTTTATACTTATGCCAATACTTTTTGAGTAAAACAAAGCAAGTGCTTATAAATAATT
AAAGCCAATTTTATAATATATATTTTTTTATTTAATTTGAATTTAGTAGTATAATTTTTT
ATGGTAAGTGCTCAAAGAGAGTTGCTTATAAAGTATGGTTTGTTTCTTTTTCGCCATTTT
GAATTACACATTAAAAATATATAGATACATATATTATAATATGAAATCATTAATAATTTA
GGGAAATTCTACAAATTTAAAAACGAATAAAATAATTGTTTTTCATCATGCCATAACACA
ATATTGATATATACATGTACAAACATTTTTTTTATTTGGAAAATATAAATTATATAAAAA
AAAATGTATAGTATACAAAATGAGCATATTCACACGGGGTGGACGTTCATTTTTTCATTT
TTCCCCTGTTTTTTATGAGTATATGATAAAATTTTATGAACATTTACACAAAATGAAAAT
GGATATATAGGAAAAATGGAGCGGTATTTCATTTATCTTTGATTGTCATTTGGATATTAT
ATTACCYTGGGTAGGCAATTAAAAATGTTAAATAACAATTTAAGGAAATTATATTTTATA
TATTAAAATTAACACTGTATTATATGATTCGCTTATAAAAGCCACTCTTTCCCCATGCAA
AGCTGTTTAATATCAATTTTAACAAATTACACACATGTTAATATATTTATATATATAATT
TATATATTTATAATTTATATATTTATATTTTTATTATTTATATATTTATTATTTATTGTG
TGTGTCAATTCGGGTAGGATATACCTCTTTTTTATTGTTTAAAGCGATTTGTATTCTAAA
ATATAAAGRATTTGAAAAAGAGAAAGATAGAATATGATCCCATCATATATAGCCCTATAA
TTTTTATTTAGCAGCGAATTAATTTTTCTATTAAGTTTATGTGTAATTAAAATAACGGAA
TATATATAATACAATAAAAAAGTGCATAAATTAAAATTTTTTCAATTAAATTTTTTTTTT
TAAGGGGTTATATAATATTAAATATATAAAATACGATTATATATTTTTGCTACAATTTTT
TATATTAAGATATAAATAGTAAATAAATGGTATTATATGGCATGTAATATATAAATTTTT
TCCAATTTTTATTTTATATACACTTTTCCTTTTTTTGTCATAAAACTTAAACAATTTACA
CATTCATTTTAAAAATTGACTATTTGTTTCAACATTTTTTGAGTTTCCGTTTTATAATAG
TATTTTCATTTGTATATTGCTTATATATATAAATACACACCTAAATGTTACAAAGGATCA
ATGCATAAACCGGTGTGTCTGGTCGTCGCGATGACCCCCAAGAGGGGCATCGGCATCAAC
AACGGCCTCCCGTGGCCCCACTTGACCACAGATTTCAAACACTTTTCTCGTGTGACAAAA
ACGACGCCCGAAGAAGCCAGTCGCCTGAACGGGTGGCTTCCCAGGAAATTTGCAAAGACG
GGCGACTCTGGACTTCCCTCTCCATCAGTCGGCAAGAGATTCAACGCCGTTGTCATGGGA
CGGAAAACCTGGGAAAGCATGCCTCGAAAGTTTAGACCCCTCGTGGACAGATTGAACATC
GTCGTTTCCTCTTCCCTCAAAGAAGAAGACATTGCGGCGGAGAAGCCTCAAGCTGAAGGC
CAGCAGCGCGTCCGAGTCTGTGCTTCACTCCCAGCAGCTCTCAGCCTTCTGGAGGAAGAG
TACAAGGATTCTGTCGACCAGATTTTTGTCGTGGGAGGAGCGGGACTGTACGAGGCAGCG
CTGTCTCTGGGCGTTGCCTCTCACCTGTACATCACGCGTGTAGCCCGCGAGTTTCCGTGC
GACGTTTTCTTCCCTGCGTTCCCCGGAGATGACATTCTTTCAAACAAATCAACTGCTGCG
CAGGCTGCAGCTCCTGCCGAGTCTGTGTTCGTTCCCTTTTGTCCGGAGCTCGGAAGAGAG
AAGGACAATGAAGCGACGTATCGACCCATCTTCATTTCCAAGACCTTCTCAGACAACGGG
GTTCCCTACGACTTTGTGGTTCTCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCG
GAACCGAGCAACGCAATGAGCTCCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGG
TTGCAGGCTCCTTCTTCGGCCGCAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAA
GACCGGAAAAAACGCGAGCAAAAGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGA
GGCCATGAAGAGTTCCAGTACCTTGATCTCATTGCCGACATTATTAACAATGGAAGGACA
ATGGATGACCGAACGGGCGTTGGTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCG
CTGGATCAGGCCTTTCCACTTCTCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAA
GAGTTGCTGTGGTTCATTCGCGGCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTG
AAGATCTGGGACAAGAATGTGACACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGA
GAGGTCGGAGACATCGGCCCGGGCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATAC
AAAGACATGCACACAGACTACACAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAG
ATGCTGAGAACGAATCCAACAGATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCG
CTGGACGAAATGGCGCTGCCGCCTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAG
AAGGAGCTGTCGTGCATCATGTATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTC
AACATCGCTTCCTATTCGCTTTTGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCT
AAGGAGTTCATTCACTTCATGGGGAACACGCATGTCTACACGAACCATGTCGAGGCTTTA
AAAGAGCAGCTGCGGAGAGAACCGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAA
CGCATCAAGGAAATCGACGATTTCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCG
CACGGACGAATCCAGATGGAGATGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCC
CGTTTTTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTAAAAATGTGTA
TGTTGTGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAAAATTATGAAC
ATTTTATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTAGTATGAATAG
CCATATTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAATTTAGATATTT
ATGGAACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTATTATTTTTAC
TGTTATAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGATTTTTAAAATG
TTTATAATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATA
AACACAAATGATGTTTTTTCCTTCAATTTCGATATCGAATTCAAACAGATATTAATAGTA
TTCATAAAATGATTGACGATTTAATAAAACATAAAGATGTTGGATCTCTAAAAACTAGTA
TATTAGAAAATTTGATTGATTTAAATATATTAAATAATAGAAATAATAGGGTTCGAGATA
ATTATTTGAATATATTACATTTAGAAATATTTTCTTTATTTTCTTTTGGGCCATATCATA
ATATATATAATTTATTAGACGAATTATTTTTATATGATAATAAATTATTAAACTGGTTCA
AAAGATGGATAATATTAAATAAGAATACATATTATAATGATAATAATAATTTTAATCAAT
TGCTATTTATATCTTCTATAAAATATATGATAAAAACATTGAGTATGAAAATTAGTAAAA
AAACATTAACAAAAAAATACTTCAAATATATACCTAAATTATACAACAAAAAAAGAGCCA
GAAGTATAGACTTAAGGTTTGAAGAAATTAAACCAAATTCAATTTTAAAAAATGAAATTG
ATTACAAAAAAGAAAATTACGATAGTGATATGGGTAAAATTTTTTTAAATCGTGTCAAAG
AATATAAAATTAGTTCTAAACTTCGAGATAAAATTTTAGAAGAATTAGACAAATATAAAT
TCTCGATATCATTAGACCGTTATAAACAAGATTATTATTTTTTATCTTTAGTAGGTTTAG
GTTATTCATATATTAACAAATTTAGTGAATTAAAGTTAGGCCTATATAAATCTGTTAATA
TTATATTAAAAAAAATGTATAATATATTTTATGTTACTGAATTAATAGAAACTGTAAATG
TATTTGTAAATGTGATTGTAAATATTTATAAAAACATATTTTATAATAATAGTAAATATA
TAATTGAAAATGGAAAAATAAAATTAAGTAAAGAAAAAAAAGAAGAATTAGCTAAATTTA
TCTTATTAGAAGAAAATATAAACATCGATTGTCTACATAATCACATTTACAAAGGGCATA
AGTTTTTTACTCATGATAATTTTGATGATACAAATTATTCAGCGGCCGCCACCGCGGTGG
AGCTCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAA
CGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCT
TTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC
AGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTG
GTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTC
TTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTC
CCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGT
GATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAG
TCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCG
GTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAG
CTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTG
GCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAA
ATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGA
AGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC
TTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGG
GTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTC
GCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTAT
TATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATG
ACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAG
AATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAA
CGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC
GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCA
CGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTC
TAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTC
TGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTG
GGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA
TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAG
GTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA
TTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATC
TCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAA
AGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA
AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC
CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGT
AGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC
TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAC
GATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCA
GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG
CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG
GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT
TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAT
GGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT
GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG
CGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCA
GCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGA
GTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGT
GTGGAATTGTGA
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Restriction sites to linearize plasmid |
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Partial or complete disruption of the gene | Partial |
Additional remarks partial/complete disruption |
See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | The genetic modification results in the disruption of the following gene models PB001073.00.0, PB000529.02.0 and PB000863.00.0 (= PF11_0528).
See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3). |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | AAAGGTACCAATACATTATTAATTGG |
Additional information primer 1 | L2644 (Asp718); 5' |
Sequence Primer 2 | AAAAAGCTTGTATAATCAGAATATGCG |
Additional information primer 2 | L2645 (HindIII); 5' |
Sequence Primer 3 | AAAGAATTCAAACAGATATTAATAG |
Additional information primer 3 | L2646 (EcoRI); 3' |
Sequence Primer 4 | AAAGCGGCCGCTGAATAATTTGTATCATC |
Additional information primer 4 | L2647 (NotI); 3' |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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