RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-1022
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_1034300; Gene model (P.falciparum): PF3D7_1408200; Gene product: AP2 domain transcription factor AP2-G2, putative (AP2-G2; ApiAP2)
Phenotype Gametocyte/Gamete; Fertilization and ookinete;
Last modified: 24 March 2014, 18:23
  *RMgm-1022
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 24572359
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
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The mutant parasite was generated by
Name PI/ResearcherSinha, A; Waters, AP
Name Group/DepartmentWellcome Trust Centre for Molecular Parasitology
Name InstituteUniversity of Glasgow
CityGlasgow
CountryUK
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Name of the mutant parasite
RMgm numberRMgm-1022
Principal namepbap2-g2 (-)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
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Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteReduced gametocyte production (95% reduction).
Fertilization and ookineteReduced gametocyte production (95% reduction). No mosquito transmission was observed
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of AP2-G2

Protein (function)
PbAP2-G2 belongs to the 27 Plasmodium ApiAP2 family of transcription factors

Phenotype
Reduced gametocyte production (95% reduction). No mosquito transmission was observed.

Additional information

Other mutants

  Disrupted: Mutant parasite with a disrupted gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1034300
Gene Model P. falciparum ortholog PF3D7_1408200
Gene productAP2 domain transcription factor AP2-G2, putative
Gene product: Alternative nameAP2-G2; ApiAP2
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedYes
Name of PlasmoGEM construct/vectorPbGEM-039238
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren