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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_1233600
|
| Gene Model P. falciparum ortholog |
PF3D7_0518800
|
| Gene product | secreted ookinete protein, putative |
| Gene product: Alternative name | PSOP13, putative secreted ookinete protein 13 |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
|
| Type of plasmid/construct used | Plasmid double cross-over |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
|
| Plasmid/construct map |
|
| Plasmid/construct sequence |
|
| Restriction sites to linearize plasmid |
|
| Partial or complete disruption of the gene | Complete |
| Additional remarks partial/complete disruption |
|
| Selectable marker used to select the mutant parasite | tgdhfr |
| Promoter of the selectable marker | pbdhfr |
| Selection (positive) procedure | pyrimethamine |
| Selection (negative) procedure | No |
| Additional remarks genetic modification | Partial gene model. Second gene model involved in the genetic modification: PB300830.00.0 |
| Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences 
Primer information: Primers used for amplification of the target sequences
| Sequence Primer 1 | TTGGGCCCAATAACTATCGTTTTTTACAACG |
| Additional information primer 1 | AE13a (ApaI); 5' |
| Sequence Primer 2 | CCAAGCTTTTGATGCGATACTAAATATTACG |
| Additional information primer 2 | AE13b (HindIII); 5' |
| Sequence Primer 3 | TGAATTCGTAAGATAAATATTTTGTTGCCT |
| Additional information primer 3 | AE13c (EcoRI), 3' |
| Sequence Primer 4 | GGGGATCCTTTTATACCATTCCTGAAAGATG |
| Additional information primer 4 | AE13d (BamHI), 3' |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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