RMgmDB - Rodent Malaria genetically modified Parasites
Back to search resultsSummaryRMgm-974
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Last modified: 22 December 2013, 21:33
*RMgm-974| Successful modification | The gene/parasite could not be changed/generated by the genetic modification. |
| The following genetic modifications were attempted | Gene disruption |
| Number of attempts to introduce the genetic modification | Unknown |
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 24348254 |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone | Not applicable |
| Other information parent line | |
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| Attempts to generate the mutant parasite were performed by | |
| Name PI/Researcher | C. Suarez; O. Billker; M.J. Blackman |
| Name Group/Department | Division of Parasitology |
| Name Institute | Medical Research Council National Institute for Medical Research, Mill Hill |
| City | London |
| Country | UK |
Disrupted: Mutant parasite with a disrupted gene| top of page | |||||||||||||||||||||||||
| Details of the target gene | |||||||||||||||||||||||||
| Gene Model of Rodent Parasite | PBANKA_1107100 | ||||||||||||||||||||||||
| Gene Model P. falciparum ortholog | PF3D7_0507500 | ||||||||||||||||||||||||
| Gene product | subtilisin-like protease 1 | ||||||||||||||||||||||||
| Gene product: Alternative name | SUB1 | ||||||||||||||||||||||||
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| Details of the genetic modification | |||||||||||||||||||||||||
| Inducable system used | No | ||||||||||||||||||||||||
| Additional remarks inducable system | |||||||||||||||||||||||||
| Type of plasmid/construct used | Plasmid double cross-over | ||||||||||||||||||||||||
| PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
| Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
| Plasmid/construct map | |||||||||||||||||||||||||
| Plasmid/construct sequence | |||||||||||||||||||||||||
| Restriction sites to linearize plasmid | |||||||||||||||||||||||||
| Partial or complete disruption of the gene | Unknown | ||||||||||||||||||||||||
| Additional remarks partial/complete disruption | |||||||||||||||||||||||||
| Selectable marker used to select the mutant parasite | unknown | ||||||||||||||||||||||||
| Promoter of the selectable marker | unknown | ||||||||||||||||||||||||
| Selection (positive) procedure | unknown | ||||||||||||||||||||||||
| Selection (negative) procedure | No | ||||||||||||||||||||||||
| Additional remarks genetic modification | Attempts to disrupt the pbsub1 gene in P. berghei blood stages failed (S. Yeoh, R. Tewari, O. Billker and M. Blackman, unpublished), suggesting that PbSUB1 too is indispensable in the erythrocytic parasite life cycle. No details of the unsuccessful attempts are provided. See RMgm-952 and RMgm-973 for conditional knock-out mutants of SUB1 (mutants that lack expression of SUB1 in liver stages) | ||||||||||||||||||||||||
| Additional remarks selection procedure | |||||||||||||||||||||||||
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Primer information: Primers used for amplification of the target sequences
 Primer information: Primers used for amplification of the target sequences
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