| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 32488076
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. chabaudi |
| Parent strain/line | P. c.chabaudi AS |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Subudhi AK, Pain A |
| Name Group/Department | Pathogen Genomics Group, BESE Division |
| Name Institute | King Abdullah University of Science and Technology (KAUST) |
| City | Thuwal |
| Country | Kingdom of Saudi Arabia |
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| Name of the mutant parasite |
| RMgm number | RMgm-4839 |
| Principal name | P. chabaudi sr10KO |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | All infections of wild type and sr10KO clones (sr10KOA and B) were highly synchronous (amplitude ± SE) for P. chabaudi wild type: 0.94 ± 0.02, P. chabaudi sr10KOA: 0.79 ± 0.02 and sr10KOB 0.93 ± 0.03). Period estimates for the proportion of parasites at early trophozoite stage suggest the intra-erythrocytic developmental cycle (IDC) duration of both sr10KO clones is ~2–3 h shorter (IDC duration 22.4 h) than the wild type (IDC duration 25.15; p < 0.0001). |
| Gametocyte/Gamete | Not tested |
| Fertilization and ookinete | Not tested |
| Oocyst | Not tested |
| Sporozoite | Not tested |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of SR10
Protein (function)
Seven transmembrane domain-containing receptors/serpentine receptors/G protein-coupled receptors (GPCRs) are the largest and most diverse group of membrane receptors and participate in a variety of physiological functions. The P. falciparum proteome contains four serpentine receptor (SR) proteins; SR1, SR10, SR12, and SR2534. Of these, only Pfsr10 showed a 24 h expression rhythm.
SR10 has been bioinformatically classified as a member of Class A serpentine receptors belonging to the hormonal receptor subclass based on the length of the N-terminal domain34 and classification by Inoue et al. SR10 is also the only receptor shared between malaria parasites and other apicomplexans and also with distantly related organisms such as Caenorhabditis elegans, Drosophila melanogaster, Gallus gallus, Mus musculus, Homo sapiens, and Arabidopsis thaliana (data retrieved from OrthoMCL DB), although it has not been linked to circadian clocks in these organisms.
Phenotype
All infections of wild type and sr10KO clones (sr10KOA and B) were highly synchronous (amplitude ± SE) for P. chabaudi wild type: 0.94 ± 0.02, P. chabaudi sr10KOA: 0.79 ± 0.02 and sr10KOB 0.93 ± 0.03). Period estimates for the proportion of parasites at early trophozoite stage suggest the intra-erythrocytic developmental cycle (IDC) duration of both sr10KO clones is ~2–3 h shorter (IDC duration 22.4 h) than the wild type (IDC duration 25.15; p < 0.0001). These results implicates sr10 in the control of developmental progression through the IDC.
Additional information
Evidence is presented that::
- Serpentine receptor 10 (SR10) has a 24 h transcriptional rhythm and disrupting it in rodent malaria parasites shortens the intra-erythrocytic developmental cycle (IDC) by 2-3 h
- SR10 affects rhythmic expression of spliceosome machinery
Other mutants |
*RMgm-4839
