Summary

RMgm-4439
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_0924800; Gene model (P.falciparum): PF3D7_1123500; Gene product: golgi protein 2 (GP2)
PhenotypeNo phenotype has been described
Last modified: 20 April 2018, 15:25
  *RMgm-4439
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 29579782
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherHallée S, Mair GR, Richard D
Name Group/DepartmentCentre de recherche en infectiologie
Name InstituteCHU de Québec-Université Laval
CityQuebec City, Quebec
CountryCanada
Name of the mutant parasite
RMgm numberRMgm-4439
Principal nameΔgp2
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of GP2

Protein (function)
Eveidence is provided that GP2 (Golgi protein 2) forms a complex with Golgi protein 1 (PF3D7_1320000; rhoptry protein 2, putative; golgi protein 1; PBANKA_1418300). The Golgi Protein complex localizes to the cis-Golgi throughout the erythrocytic cycle and  potentially  also  during  the  mosquito  stages.

Phenotype
GP2 is not essential throughout the complete lifecycle. No phenotypes were detected different from wildtype.

Additional information
The  extensive  colocalization  between  GP2  and  the  cis-Golgi apparatus  marker  Endoplasmic  reticulum  Retention  Defective  2  (ERD2)  confirmed that GP2 resides in the Golgi throughout the blood stages. This staining pattern was conserved in the rodent malaria parasite P. berghei, where we tagged the endogenous locus of the orthologous gene PBANKA_092480 with GFP. The tagged protein was  also  detected in  gametocytes,  as  well  as  all  mosquito  stage  parasites  including  the ookinete, midgut oocysts, and sporozoites providing evidence for the constitutive expression of GP2 throughout the life cycle of the parasite. In addition, the staining pattern of PbGP2 in the mosquito stages resembled what was previously described with PbGP1 which suggests that they might perhaps also form a complex in this phase of the life cycle

Other mutants
4438: a mutant expressing a C-terminal GFP-tagged version of GP2


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0924800
Gene Model P. falciparum ortholog PF3D7_1123500
Gene productgolgi protein 2
Gene product: Alternative nameGP2
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markerunknown
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6